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Nasution, Rumondang Bulan
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Fabrication of Bioethanol via Fermentation from Cellulose of Kepok Banana (Musa Paradisiaca L.) Using Bread's Yeast (Saccharomyces cerevisiae) Naufal, A. Dzaki; Nasution, Rumondang Bulan
Journal of Chemical Natural Resources Vol. 4 No. 2 (2022): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v4i2.11964

Abstract

Bioethanol has been made by fermentation of the cellulose of Kepok banana stems, where this study went through 3 stages. First-stage isolation of cellulose from Kepok banana prevents using HNO3 and NaNO2, removal of swelling with NaOH and Na2SO3, and bleaching process (bleaching) using NaOCl and H2O2, then functional group analysis was performed with FT-IR. The second stage of cellulose obtained was hydrolysed with 1% HCl for 120 minutes of hydrolysis time. The resulting glucose was analysed by Benedict's reagent and the Luff-Schrool method. The third stage of glucose solution results from fermented hydrolysis using bread yeast (Saccharomyces cerevisiae) with variations in the weight of yeast 5, 6, and 7 grams, and the duration of fermentation is five days. Bioethanol obtained was tested qualitatively using K2CrO7 and H2SO4 reagents and bioethanol characterisation using GC. The study results obtained 10.18% glucose levels, and the highest levels of bioethanol were obtained at 11.27% with 88.53% purity using 7 g of yeast.
Utilization of Carboxymethyl Cellulose (CMC) from Coconut Coir Waste (Cocos nucifera) as a Stabilizer in Red Bean (Phaseolus vulgaris L) Vegetable Milk Nasution, Rumondang Bulan; Simanungkalit, Roberto Carlos
Journal of Chemical Natural Resources Vol. 5 No. 1 (2023): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v5i1.11988

Abstract

Carboxymethyl cellulose (CMC) synthesis from coconut coir waste provides an alternative that can be used as a stabilizer to manufacture red bean milk. The first step is to isolate alpha-cellulose with a 2% NaOH solution. Second, the etherification process is alkalization with isopropanol and 17.5% NaOH solution to produce alkaline cellulose, and then the carboxymethylation process with sodium monochloride acetate (NaMCA) and characterized by FT-IR. The resulting carboxymethyl cellulose is purified by centrifugation. Red bean milk was analyzed using proximate analysis, viscosity, percent stability, pH, and organoleptic content by varying CMC 0.1, 0.2, and 0.5%. The alpha cellulose obtained was 13.37%, and the CMC produced from the etherification and purification process was 5.97 g. The characterization of alpha-cellulose showed (OH) at wave number 3441cm-1, and the CMC characterization showed carbonyl (COO-) at wave number 1604.77 cm-1 and group ether (CH2-O-CH2) at wave number 1419.77. Proximate analysis of red bean milk protein content of 2.75%, carbohydrates 10.27%, fiber 8.5%, and fat 18.57% and results based on viscosity and percent stability were obtained by adding 0.5% CMC and organoleptic test results adding CMC preferably with 0.3%, the texture is not too thick and even, and the aroma of red beans is typical.
Co-Authors Naufal, A. Dzaki Simanungkalit, Roberto Carlos