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Jansen, Feky
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Biochemistry, Genetics & Molecular Biology Chemistry Engineering Environmental Science

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Extraction And Identification Of Sulfated Polysaccharide From Gracilaria sp. Malle, Dominggus; Fransina, Eirene G.; Jansen, Feky
Indonesian Journal of Chemical Research Vol 1 No 2 (2014): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2014.1-dom

Abstract

Polysaccharide from Gracilaria sp (locally known as “Sayur Karang”) was extracted using hot alkaline solutions (NaOH and KOH). Fractionation using DEAE-Sepharose Fast Flow column showed that the polysaccharide discharged after being eluted with 0.5 – 1.0 M NaCl. Spectroscopic analysis using FTIR showed that the polysaccharide contained hydroxyl (3420 cm-1 (NaOH frcation) dan 3446 cm-1 (KOH frcation)), carbonyl (1642 cm-1 (NaOH frcation) and 1644 cm-1 (KOH frcation)) and sulphate (1271 cm-1 (NaOH frcation) and 1262 cm-1 (KOH frcation)) groups proving that the polysaccharide is a sulphated polysaccharide. A further study is needed to elucidate the structure of the sulphated polysaccharide as well as physiochemical properties of the polysaccharide.
Co-Authors DOMINGGUS MALLE Fransina, Eirene G.