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All Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya IJCR (Indonesian Journal of Chemical Research)
Wulandari, Eka Yunita
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Materials Science & Nanotechnology Medicine & Pharmacology

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Sintesis dan Karakterisasi Nanopartikel CuO-Kitosan serta Uji Antibakteri terhadap Streptococcus pneumoniae Qomariyah, Ani; Wulandari, Eka Yunita; Najib, Ainun
INDONESIAN JOURNAL OF CHEMICAL RESEARCH Vol. 10 No. 1 (2025): Volume 10, ISSUE 1, 2025
Publisher : Chemistry Department, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijcr.vol10.iss1.art2

Abstract

The green chemistry technique was carried out for the first time to synthesize CuO-Chitosan nanoparticles using CuSO4 as a precursor and red dragon fruit peel extract as a reducing agent which was then applied as an antibacterial agent. The extraction procedure of red dragon fruit peel was carried out by maceration technique with methanol solvent. CuO-chitosan nanoparticles were produced through green chemistry with reduction method. The compounds in the red dragon fruit peel extract reduced Cu2+ to Cu. The successfully synthesized Cu-NPs were analyzed using FT-IR, SEM, PSA, and UV-Visible Spectrophotometer. The variation of reducing agent volume affected the maximum wavelength produced. The optimum reducing agent concentration (1:5) showed the highest intensity at 650 nm. The optimum pH for CuO-Chitosan synthesis was 6. Cu-NPs were stable at 20 minutes to 1 hour. Identification of functional groups with FTIR showed a shift in wave numbers between pure CuSO4 precursor and CuO-Citosan Nanoparticles, indicating the interaction of functional groups. Volume or weight analysis of CuO-Citosan Nanoparticles using PSA gave particle diameters between 80 and 120 nm with an approximate distribution of 100 nm. The results of the antibacterial test showed that the growth of Streptococcus pneumoniae bacteria produced the highest inhibition zone in CuO-Citosan Nanoparticles with a concentration of 100% which had an average inhibition zone diameter of 11 mm. This proves that CuO-chitosan nanoparticles are effective against Streptococcus pneumoniae. Keywords: CuO-chitosan, nanoparticles, Streptococcus pneumoniae
Escherichia coli and Salmonella sp. on some lettuce in the traditional market of Banyuwangi Regency Wulandari, Eka Yunita; Ayu, Sinta Nuriyah Putri; Hanifa, Nur Lailatul; Handayani, Brunika Mega; Fisabilla, Adhinda
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 8 No 01 (2024): Qanun Medika Vol 08 No 01 January 2024
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v8i01.17265

Abstract

Escherichia coli is the main pathogenic bacteria which causes diarrhea in humans and the fourth highest cause of death which occurs all age groups. Salmonella sp is the cause of typhoid fever and findings of 350-810 cases each year. Diarrhea and typhoid fever are closely related to the consumption of raw vegetables, such as lettuce. This study aimed to analyze the contamination of E. coli and Salmonella sp on some lettuces in Traditional Markets of Banyuwangi Regency. This research was an analytic descriptive study using a cross-sectional design at the Laboratory of Microbiology, STIKES Banyuwangi from 6 to 11 June 2022 using 6 lettuces as research samples which were selected by accidental sampling. All (100%) lettuce samples in this study were contaminated with >1,100 CFU/ml E. coli. All (100%) samples in this study were positively contaminated by Salmonella sp with various contamination number, such as: 0.82x104; 0.47x104, 1.90x105, 2.05x106, 1.00x105 and 1.25x106 CFU/ml, respectively. All six lettuces obtained from the Traditional Market of Banyuwangi Regency did not meet the microbiological quality specified by SNI, indicating that they were not suitable for  direct (raw) consumption.
Co-Authors Afifah Ainun Najib Ayu, Sinta Nuriyah Putri Fisabilla, Adhinda Handayani, Brunika Mega Hanifa, Nur Lailatul Qomariyah, Ani