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Sari, Ria Yustika
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah Choirunnisa, Hesti Nur; Sari, Ria Yustika; Hastuti, Utami Sri; Witjoro, Agung Witjoro
bionature Vol 18, No 2 (2017): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.895 KB) | DOI: 10.35580/bionature.v18i2.6138

Abstract

Abstract. Wadi is a sort of traditional foods that made of fish from Dayak tribe in Central Kalimantan. Wadi is made by the addition of salt and lumu. People make wadi as a supply of side dishes during difficult seasons of fish and stock for farming, hunting, or collecting forest products. In wadi, there are several bacteria species that capable in degrading amylum and protein. The bacteria capability in amylum and protein degradation can be determined by calculating the hydrolysis index. The aims of this study are to: (1) identify the amylolytic bacteria and proteolytic bacteria species from wadi, (2) determine the amylum and protein hydrolysis index on each amylolytic bacteria and proteolytic bacteria species isolated from wadi, and (3) determine the amylolytic bacteria and proteolytic bacteria species that have the highest hydrolysis index. This research is descriptive explorative research. The research results showed that: (1) there were four isolates of amylolytic bacteria isolated from wadi, i.e: Enterobacter agglomerans, Pseudomonas fluorescens, Acinetobacter baumannii, and Micrococcus varians, and there were five species of isolated proteolytic bacteria from wadi, i.e: Enterobacter agglomerans, Pseudomonas fluorescens, Nitrococcus mobilis, Acinetobacter baumannii, and Micrococcus varians, (2) the amylum hydrolysis index of Enterobacter agglomerans is 3.77; on Pseudomonas fluorescens is 3.00; on Acinetobacter baumannii is 4.16; and on Micrococcus varians is 7.23. The protein hydrolysis index of Enterobacter agglomerans is 3.22; on Pseudomonas fluorescens is 2.25; on Nitrococcus mobilis is 1.67; on Acinetobacter baumannii is 3.42; and on Micrococcus varians is 3.45, (3) Micrococcus varians has the highest amylum, i.e:  7.23 and protein hydrolysis index is 3.45.Keywords: hydrolysis index, amylolytic bacteria, proteolytic bacteria, wadi.
Observasi Histologik dan Identifikasi Fungi Endofit yang Diisolasi dari Cananga odorata (Lam.) Hook.F. & Thomson Hastuti, Utami Sri; Rakhmawati, Dwi; Sari, Ria Yustika
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

Some endophytic fungi live in medicinal plant tissue, include medicinal plant. This research was done to: 1) determine the endophytic fungi position in the Cananga odorata  plant tissue by histological observation; 2) identify the endophytic fungi species isolated from the leaf, twig, and petal flower of C. odorata. The position determination of endophytic fungi in the C. odorata plant tissue is done by microscopic observation. The endophytic fungi were isolated from the leaf, twig, and flower petal of C. odorata  plant by cutted these plant parts, then inoculated on Potato Dextrose Agar (PDA) medium and incubated in 27oC during 7 x 24 hours, each fungi that grow on the PDA medium were isolated, then the morphology and microscopic characteristic of each fungi colony isolates were described. Each endophytic fungi isolates were identified. The research result were:  1) the endophytic fungi position are on the epidermis cell wall and on the stomata guard cell wallof the leaf tissue, on the parenchymal cell wall of the twig tissue, and on the epidermis cell wall of the flower tissue; 2) Nine endophytic fungi species were isolated from the C. odorata leaf, twig, and flower petal are: Nigrospora sphaerica, Colletotrichum alienum,Mycellia sterilia1, C. kahawae, Rhizoctonia sp., C. aotearoa, Micellia sterilia2, C. alatae, dan C. queenslandicum
Co-Authors Agung Witjoro Choirunnisa, Hesti Nur Dwi Rakhmawati Utami Sri Hastuti