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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Alchemy : Journal of Chemistry Agrointek Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology)
Rifa'i, Muhaimin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment Suprihatin, Teguh; Widyarti, Sri; Rifa'i, Muhaimin; Rahayu, Sri
Journal of Tropical Life Science Vol 9, No 3 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Premenopause is a physiological condition in a female individual that has entered the aging period, a condition usually characterized by elevated MDA levels and decreased estrogen levels. The objective of this study was to determine the level of ovarian MDA and estradiol serum levels of premenopausal white rat blood after oral turmeric powder treatment. The animals used were 30 female Wistar strains white rat, age 12 months with an average body weight 200-250 g. The animals were divided into 6 groups, namely the negative control group (P0) with 4 ml/day distilled water treatment; positive control group (P1), this group was treated with pure curcumin powder 6.75 mg/kg BW; treatment group 1 (P2), group was treated with turmeric powder 100 mg/kg BW; treatment group 2 (P3); treatment group 3 (P4); and treatment group 4 (P5), these group were treated with turmeric powder at 200 mg/kg BW; 400 mg/kg BW; and 800 mg/kg BW dose respectively. Oral Treatment was administered daily for 27 days. Blood collection was performed on days 0, 14, and 28. The ovarian collection was conducted on day 28. MDA ovarian level was measured using TBA method and blood serum estradiol level was measured using ELISA method. The results exhibited that the positive control group (P1) and the treatment group (P2-P5) showed significantly lower ovarian MDA levels compared with the negative control group (P0). The turmeric powder dose 200 mg/kg BW (P3) can increase estradiol levels by day 14 (3.32 ± 0.26 ρg/mL) and at day 28 (4.01 ± 0.26 ρg/mL).
Modulation of Granulocyte Cells Development by VipAlbumin® Administration in BALB/C Mice with Diabetes Mellitus Adi Pradana, Andi Rizki; Ibrahim, Mansur; Sasmito Djati, Muhammad; Rifa'i, Muhaimin
Journal of Tropical Life Science Vol 5, No 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.05

Abstract

Diabetes mellitus is a metabolic disease that is caused either by the decrease of insulin secretion frompancreatic β cells or the insensitivity of target cells against insulin. High glucose levels (hyperglycemia condition)can trigger the formation of free radicals, the main cause of diabetes micro and macrovascular complications. Theformation of free radicals and AGE (advanced glycation end-products) is assumed to became the key factor in thedecline of granulocyte cell production as well as the disruption of these cells functional activity. The purpose ofthis research was to determine the role of VipAlbumin® in inhibiting the adverse effects of increased blood glucoselevels, which highly influence the production of granulocyte. This study was divided into in vitro and in vivostage. BALB/C mice were used as experimental animals at in vivo stage and induced to undergo diabetes through100 mg/kg BW streptozotocin (STZ) injection at the age of 5 days. VipAlbumin® administered orally for 14 days,which began when mice reached the age of 14 weeks. The administration of VipAlbumin® divided into 3 dosesi.e. 0,01664 mg/gr BW (1st dose), 0,416 mg/gr BW (2nd dose), and 10,4 mg/gr BW (3rd dose). The further step wasa flowcytometric analysis to see the development of granulocyte cells relative amount, which were isolated fromthe bone marrow. The result of this analysis shows that VipAlbumin® administration, particularly at the 2nd and3rd dose, were able to modulate granulocyte cells development in the bone marrow.
Gynura procumbens Ethanolic Extract Promotes Lymphocyte Activation and Regulatory T Cell Generation In Vitro Dwijayanti, Dinia Rizqi; Rifa'i, Muhaimin
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

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Abstract

Immune system is a system of biological structures and processes within organism directed to protect against invaded pathogen. Cellular and humoral immune system mediated by immunocompetent cells such as CD4+ T cells, CD8+ T cells, CD4+CD25+ T cells, and B220 cells play important role for maintaining immunological surveillance. The purpose of this study was to determine the effect of ethanolic extract of  G. procumbens leaves (EEGL) on the profile of CD4+ T cells, CD4+CD25+ T cells, and B220+ cells. Splenic cells were isolated from BALB/c mice and cultured in RPMI1640 medium in the presence of EEGL. After 4 days of incubation, cells were harvested, stained with antibodies and analyzed by flow cytometer. The data were analyzed by one-way ANOVA with α= 0.05 and Tukey test using SPSS 16.0 for windows. The results showed that the extract of  G. procumbens could increase proliferation of CD4+CD62L T cell, CD4+CD25+T cells, and B220+ cells compared to the control. Here, we showed the biological effect of G. procumbensas medicinal herb with immunomodulatory activity andthe dose of 0.1 µg/ml and 1.0 µg/ml could promote T cell activation compared to the highest dose of 10 µg/ml. In terestingly, the dose of 10 µg/ml rather promote than inhibit B cell proliferation.
Immunomodulatory Activity of Methanol Leaf Extract of Neem (Azadirachta indica Juss) Against Suppressor and Proinflammatory Molecules Supriyanto, Supriyanto; Widjanarko, Simon Bambang; Rifa'i, Muhaimin; Yunianta, Yunianta
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.07

Abstract

Neem plant is rich in bioactive constituents, which make it massively discussed the treatment of various diseases. A study on the immunomodulatory activities of neem is given here. This current work aimed to investigate the effects of neem leaf extract on immunocompetent cells. In vivo experiment was carried out using mice (Mus musculus)  induced with DMBA, comprising positive control, negative control, and treatments of neem leaf extracts (250, 500, and 1000 ppm). Data obtained from flow cytometric analysis were evaluated using BD Cellquest ProTM software, then statistically analyzed in SPSS version 21. Parametric analysis in one-way ANOVA was performed at a significance level of 5%. The significant difference was compared in the Duncan test. The results showed that administration of neem leaf extracts significantly affected the expression of CD4+, CD8+, CD25+, CD62L, IL-10, and IL-17 cells .Neem leaf extract has immunomodulatory activities by increasing pressure molecules and decreasing pro-inflammatory molecules
Co-Authors A.A. Ketut Agung Cahyawan W Andi Rizki Adi Pradana, Andi Rizki Dinia Rizqi Dwijayanti Dr.Ir. Yunianta, DEA Ihwan Ihwan Loeki Enggar Fitri Mansur Ibrahim Moch Sasmito Djati SIMON BAMBANG WIDJANARKO SRI RAHAYU Sri Widyarti Supriyanto Supriyanto Teguh Suprihatin