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All Journal Microbiology Indonesia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
Agus Syahrurachman
Departemen Mikrobiologi Klinik Fakultas Kedokteran Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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DAYA ANTI BAKTERI KOMBINASI FOSFOMISIN DAN SULBAKTAM-SEFOPERAZON TERHADAP BAKTERI PSEUDOMONAS AERUGINOSA Agus Syahrurachman; Fera Ibrahim; Atna Permana
Majalah Kedokteran Indonesia Vol 69 No 12 (2019): Journal of The Indonesian Medical Association Majalah Kedokteran Indonesia Volu
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/jinma.v69i12.164

Abstract

Pendahuluan: Pseudomonas aeroginosa dapat merupakan penyebab infeksi diberbagai organ. Pilihan antibiotika tunggal untuk itu menjadi lebih sedikit.Tujuan: Menilai daya antibakteri kombinasi fosfomisin dan sulbaktkam-sefoperazon in vitro terhadap isolat klinis Pseudomonas aeruginosa.Metode: Uji kerentanan 30 isolat klinis Pseudomonas aeruginosa terhadap fosfomisin dan sefoperazone-sulbactam sebagai obat tunggal dan kombinasinya dilakukan pada media cair. Daya antibakteri kombinasi fosmisin dan sulbactam sefoperazon ditentukan dengan menghitung Fraction Inhibition Index (FIC). Time-kill curve dinilai pasca paparan bakteri terhadap antibiotika selama waktu tertentu. Hasil: Proporsi isolat yang sensitif sulbactam-sefoperazon sebanyak 66,7% dan meningkat menjadi 90% terhadap kombinasi antibiotika. Proporsi isolat yang sensitif fosfomisin hanya 10% dan meningkat dua kali lipat terhadap kombinasi antibiotika. Efek sinergis, indifferent dan antagonis masing-masing ditemukan pada 60%, 36.7% dan 3.3% isolat. Dari curva time kill disimpulkan bahwa kematian bakteri yang cepat terjadi dalam 4 jam pertama. Kesimpulan: kombinasi sulbaktam sepoferazon dan fosfomisin pada sebagian besar isolat Pseudomonas aeruginosa bersifat sinergis dan hanya 1 galur yang menunjukkan efek antagonis.
Daya Anti Bakteri Kombinasi Fosfomisin dan Sulbaktam-sefoperazon terhadap Bakteri Pseudomonas aeruginosa Agus Syahrurachman; Fera Ibrahim; Atna Permana
Majalah Kedokteran Indonesia Vol 69 No 12 (2019): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, V
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.69.12-2019-164

Abstract

Introduction: Pseudomonas aeruginosa may cause infections in various organs. Due to increase resistance, single antibiotic option for treatment of those infections is more limited.Objective: to elcucidate in vitro antibacterial effect of fosfomycin and sulbactam-cefoperazone alone and its combination against clinical isolates of Pseudomonas aeruginosa.Methods: Minimal Inhibitory concentration of fosfomycin and cefoperazone-sulbactam as a single drug and their combination was determined in liquid media against 30 clinical isolates of the Pseudomonas aeruginosa. Effect of antibiotic combination was calculated by Fraction Inhibitory Index. Time-kill curve was plotted according to the number of viable bacteria after antibiotic exposure.Results: The proportion of susceptible isolate to sulbactam-cefoperazon alone was 66.7% and increased to 90 % to combined antibiotics. Proportion of susceptible bacteria to fosfomycin alone was 10 % and increase to 20 % to combined antibiotics. Synergistic, indifferent and antagonistic effects were found in 60%,36.7% and 3.3% strains respectively. Rapid bacterial death occurred within the first 4 hours of exposure.Conclusion : Combination of sulbactam-cepoferazone and fosfomycin have sinergism in majority of tested strain of Ps aeruginosa and only one strain show antagonistic effect.
Levels of CXCL10 Chemokine in Dengue Infected Hepatocyte Huh 7 it-1 Cell Line Co-cultured with Peripheral Blood Mononuclear Cells BETI ERNAWATI DEWI; EVA DAMAYANTI; TJAHJANI MIRAWATI SUDIRO; AGUS SYAHRURACHMAN
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.52 KB) | DOI: 10.5454/mi.13.3.4

Abstract

Dengue is a mosquito borne virus that spreads rapidly in the world. At present, it is estimated that more than 3.9 billion people are at risk of being infected with dengue virus (DENV) and there are 96 million clinical cases that have been reported annually in 128 countries worldwide. In DENV infected patients often associated with liver dysfunction which hepatocyte and kuppfer cells as the main target of viral infections. DENV infection induced the expression of several chemokines, which might play an important role during the inflammatory response and pathogenesis of a disease. CXCL10 is known as a chemokine that activates lymphocytes for innate and adaptive immunity, induces tissue damage, and modulates tumor formation. Therefore, we conducted an in vitro study using Huh 7it-1 cells co-cultured with peripheral blood mononuclear cells (PBMCs) to investigate CXCL10 chemokine induction during DENV infection. Huh 7it-1 cells were grown on 96 micro well plate until a monolayer was formed. The cells were infected with DENV-2 at an MOI of 0.5 FFU/cell and 1 FFU/cell in the presence of PBMCs. Heat inactivated DENV-2 and Huh7 cell medium were used as control. After 2 hours of infection, cells were co-cultured with PBMCs and incubated at 37 ºC with 5% CO 2 for 48 h. Cell supernatant was collected and CXCL10 chemokine levels were measured using CXCL10 Quantikine ELISA Kit. Statistical analysis was performed by SPSS 23. In the presence of PBMCs, CXCL10 levels from DENV infected Huh 7it-1 at an MOI of 0,5 FFU/cell and MOI of 1 FFU/cell were 552,653 ± 22,779 pg  mL-1 and 576,787 ± 16,901 pg  mL-1 . Those levels were higher when compared with supernatan from heat inactivated DENV-2 and control cells. Without PBMCs, all of treatments showed lower level of CXCL10. DENV-2 infection in Huh 7it-1 cells co-cultured with PBMCs was able to induce CXCL10 secretion. Furthermore, heat inactivated DENV-2 also still capable to inducen the secretion of CXCL10 chemokine in Huh 7it-1 cells.
Sinergisme In Vitro Sulbactam-Cefoperazone dan Fosfomycin Terhadap Escherichia Coli dan Klebsiella Aeromobilis Di Indonesia Agus Syahrurachman; Atna Permana
Majalah Kedokteran Indonesia Vol 71 No 5 (2021): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.71.5-2021-546

Abstract

Introduction: There is no susceptibility data of E. coli and K. aeromobilis in Indonesia, even data regarding minimal inhibitory concentration (MIC)-based susceptibility of E. coli and K. aeromobilis towards single antibiotic or combination of fosfomycin (FOS) and sulbactam-cepoferazone (SUL-CPZ) is very scarce, even though the data is required by clinicians. Methods: A descriptive observational study was carried out at the Microbiology Clinical Laboratory of the Faculty of Medicine, Universitas Indonesia. Thirty strains each of clinical isolates of E. coli and K. aeromobilis were subjected to MIC determination against FOS and SUL-CPZ. For susceptibility criteria, we adopted the Eucast guideline. The synergism of the combined antibiotics was determined by checkerboard titration. One strain of E. coli and K. aeromobilis showing a synergistic and independent effect against the combined antibiotics was subjected to a time-kill assay. The post-antibiotic effect (PAE) was determined on a strain of E. coli showing synergism against the combined antibiotics. Results: The MIC level of all strains decreased when the bacteria were exposed to the combined antibiotics. Synergism was observed in 53.3% of E. coli and 56.8% of K. aeromobilis. No antagonism was observed. Higher bacterial death during the first four hours occurred with the isolate, showing synergism compared to the isolate showing an independent effect. The PAE of E. coli was longer when exposed to combined antibiotics. Conclusion: In vitro synergism of FOS and SUL-CPZ was observed in the majority of isolates and could be used as the basis for further research on empirical treatment
Co-Authors Atna Permana Atna Permana Atna Permana Beti Ernawati Dewi Elfrida Ratnawati EVA DAMAYANTI Fera Ibrahim Tjahjani Mirawati Sudiro