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All Journal Jurnal Sains dan Seni ITS IPTEK Journal of Proceedings Series IPTEK The Journal for Technology and Science Biota Sewagati
Kuswytasari, Nengah Dwianita
Departemen Biologi Institut Teknologi Sepuluh Nopember
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Environmental Science Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : IPTEK The Journal for Technology and Science

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Antimicrobial Assay of Soil Mold Isolates from Wonorejo Surabaya Arisanti, Septia; Kuswytasari, Nengah Dwianita; Shovitri, Maya
IPTEK The Journal for Technology and Science Vol 23, No 4 (2012)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.508 KB) | DOI: 10.12962/j20882033.v23i4.100

Abstract

This study was aimed to an examine antimicrobial activity of 34 soil molds isolates from the Wonorejo Surabaya on the growth of Gram negatif bacteria (Escherichia coli and Coliform Bacteria Group), Gram positif bacteria (Bacillus subtilis) and yeast (Saccharomyces cerevisiae). Antimicrobial ability detected with modification of dual culture antagonism assay in Potato Dextrose Agar (PDA) medium. The result showed that genus Aspergillus, Scopulariopsis, Penicillium, Paecilomyces, Fusarium, and Trichoderma were able to inhibit E. coli; while genus Aspergillus, Scopulariopsis, Penicillium, Paecilomyces, Exophiala, Stachybotrys, and Acremonium inhibit B. subtilis; further on only genus Aspergillus could inhibit group of Coliform bacteria); and genus Scopulariopsis, Penicillium, Trichoderma, and Absidia inhibited the growth of yeast S. cerevisiae.
Ligninolytic Enzymes Produced by Gliomastix sp. in an Organic Waste Medium Nengah Dwianita Kuswytasari; Maya Shovitri; Enny Zulaika
IPTEK The Journal for Technology and Science Vol 26, No 1 (2015)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v26i1.577

Abstract

Ligninolytic enzymes are extracelluler enzymes that have many benefit on multiple industrial department. Its can result from Gliomastix sp. This study was aimed to determine the effect of temperature and pH on the activity of Ligninolytic enzymes by Gliomastix sp. T3.7 that grow on multiple organic waste . Ligninolytic enzymes was measured with spectrofotometer UV-Vis Boeco S22. The result showed that Gliomastix sp. T3.7 potentially produce the ligninolytic enzymes like as laccase, LiP and MnP. The optimum laccase activity ( 1,765 U/ml) detected on sugarcane baggase waste, pH 6 and temperature of 350C . The optimum LiP activity (8,088U/ml) detected on corn cobs, pH 5 and 350C, whereas the optimum MnP activity (0,964 U/ml) detected on sugarcane baggase too. Its optimum on pH 4 and 250C. Gliomastix sp. T3.7 on sugarcane bagasse, pH 6 and 350C, agensia potentially used as remediation on a variety of industry pollution.
Co-Authors Aisyah Maulida Hanum Arif Luqman Arisanti, Septia Awik Puji Dyah Nurhayati Ciptian Weried Priananda Devi Meiliawati Dini Ermavitalini Enny Zulaika Fajar Diah Puspitasari Hanum Kusuma Astuti Ima Mufidatul Ilmi Imam Wahyudi Farid Kristanti Indah Purwani Lilik Nurindah Sari Nanda Ria Wanti Nur Hidayatul Alami Nurul Jadid Nurul Syamsi Prawita Herdin Doraja Putri Paramita Sari, Noorkomala Shovitri, Maya Sri Fatmawati Syayyida Muslimah Talitha Rahma Nathania Triana Qomaria Zahrotunnisa Tutik Nurhidayati Wirdatul Muslihatin Yusriah Yusriah