Anggraheni, Yuliana Galih Dyan
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EKSPLORASI MARKA SSR TERPAUT SIFAT TOLERANSI PADI GOGO TERHADAP ALUMUNIUM Anggraheni, Yuliana Galih Dyan; Mulyaningsih, Enung Sri
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3099

Abstract

ABSTRACTThe objective of this research was to study molecular markers were linked to upland rice tolerance of Situ Patenggang x B11930F-TB2 against Al using SSR method. The plant materials were 36 lines of Situ Patenggang x B11930F-TB2 with categories tolerant, moderate, and susceptible to acid soil based on selection in East Lampung. IR6008032 line and ITA variety used as comparative control of tolerant and susceptible. The results showed that 40 primer pairs were applied to 36 lines and allegedly 3 primer pairs that are: RM205, RM257 and RM247 linked to Al tolerance. The markers location analysis on rice chromosome listed as follows: RM205 and RM257 were located on chromosome 9 and RM247 located on chromosome 12 with genetic distance 93,12 cM, 772.62 cM and 13,05 cM, respectively.Keywords: SSR marker, upland rice, aluminium tolerance, ultisols
Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice Anggraheni, Yuliana Galih Dyan; Mulyaningsih, Enung Sri; Priadi, Dody; Deswina, Puspita; Sulistyowati, Yuli; Adi, Eko Binnaryo Mei; Perdani, Ambar Yuswi; Nuro, Fiqolbi; Paradisa, Yashanti Berlinda
Jurnal Biodjati Vol 5 No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.7990

Abstract

SSR marker is one of the genetic markers widely applied in plant breeding programs. The application of molecular markers in plant breeding is meant to accelerate the selection of cross-progeny. The research aimed to identify the SSR primers polymorphism between the parent and control that linked to Al tolerance and verify the cross-progeny of five crosses. The result gained from 37 SSR primers used in this study showed that only nine primers are polymorphic. These nine polymorphic primers are RM257, RM214, RM247, RM205, RM490, RM262, RM569, RM271, and RM19. The application of polymorphic markers on five cross-progeny which have shown the same band pattern as the parents and tolerant control on the use of 9 SSR primers recorded as follows: RM257 2 lines, RM214 5 lines, RM247 5 lines, RM205 lines, RM490 13 lines, RM262 5 lines, RM569 7 lines, RM271 4 lines, and RM19 6 lines. The selected SSR primers linked to Al tolerance in this research can be used as a reference for molecular breeding strategies to develop new Al tolerance rice varieties in dryland conditions.