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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Annales Bogorienses
Farajallah, Ahmad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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RAPID DETECTION OF THE AFRICANIZED HONEY BEE: A TOOL FOR INDONESIAN ANIMAL QUARANTINE RAFFIUDIN, RIKA; BINTAR, ANIFA; WIDJAJA, M. CHANDRA; FARAJALLAH, AHMAD; PURWANTARA, BAMBANG
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.086 KB) | DOI: 10.11598/btb.2009.16.1.65

Abstract

Molecular detection methods were used to determine if Africanized Honey Bees (AHBs) are present in populations of imported Apis mellifera in Indonesia. The cytochrome b (cyt b gene) was amplified from mitochondrial DNA and digested with the BglII restriction enzyme (cytb/BglII). Two types of animal DNA extraction kits were used and found suitable for rapid preparation of DNA from A. mellifera by the Animal Quarantine facility. Results showed that all 94 colony samples from beekeepers in Java produced a 485 bp PCR product from the amplification of this gene. Two DNA fragments of 194 and 291 bp from all samples were produced after digestion with BglII. This cytb/BglII result together with the DNA sequence of cyt b showed that all collected samples of A. mellifera were the non-AHB type. Hence, this study did not detect AHB in Indonesia.Key words :  Apis mellifera, molecular detection methods, DNA, cytochrome b, mitochondrial genetics
Association between Polymorphism of β-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Sumantri, Cece; Nurhayati, Nurhayati D.; Farajallah, Ahmad; Anggraeni, A. Anggraeni
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : BRIN

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Abstract

The aims of this study were to identify the effects of polymorphism of b-lactoglobulin gene on milk yield and percentage of milk protein and fat in local sheep. A total number of 83 heads of lactating ewes raised under an extensive management at Jonggol Animal Science Teaching and Research Unit (JASTRU) of the Faculty of Animal Science, Bogor Agricultural University were studied. Research activities were carried out through some steps involving blood collection. DNA isolation, DNA amplification, and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (PCR) then genotyped by single strand conformation polymorphism technique (SSCP), it was successfully amplified a fragment length at 420 bp in b-lactoglobulin genelocated on the exon 7. The electrophoresis pattern revealed 5 types of b-lactoglobulin gene and designated as A, B, C, D, and E types. The proportion for those respective 5 type from the highest to the lowest were for A (27.71%), C (16.87%), D (12.05%), E (10.84%), and B (9.64%), respectively. There were no significant effect of polymorphisms in b-lactoglobulin gene on both milk yield and quality in local sheep at JASTRU.
Co-Authors Anggraeni, A. Anggraeni Bambang Purwantara BINTAR, ANIFA Cece Sumantri Nurhayati, Nurhayati D. RIKA RAFFIUDIN WIDJAJA, M. CHANDRA