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V.A. Parthasarathy
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DESIGN AND ANALYSIS OF COCONUT EMBRYO CULTURE EXPERIMENTS Anitha Karun; K. Muralidharan; K. K. Sajini; V.A. Parthasarathy
International Coconut Community Journal Vol 19 No 01 (2003): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v19i01.366

Abstract

The experimental design and data analysis procedures followed for standardizing the CPCRI protocol of coconut zygotic embryo culture are described. The availability of human resources (for initiating the cultures as well as periodic sub-culturing), laboratory space for maintaining culture, infrastructure for preparation of adequate culture media etc, are the important factors to be considered at the planning stage. Since the response of interest per experimental unit is discrete (germinated or not), it is desired to have a “plot size” of 20. Based on the experimental errors obtained for various treatments, attempts were made to obtain the replication size for a specified value of treatment difference to be tested. In most of the experiments, per cent germination was not following the assumption of ANOVA , even after transformation. In such situation, the chi-square test was employed to study the influence of treatments on germination. The coefficient of variation in most of the experiments was below 20 %.
Cryopreservation of mature coconut embryos by desiccation method Anitha Karun; K.K. Sajini; V.A. Parthasarathy
International Coconut Community Journal Vol 21 No 1 (2005): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v21i01.395

Abstract

Mature embryos of West Coast Tall variety of coconut could be cryopreserved after desiccation pretreatments and retrieved into plantlets. The desiccation pretreatment and optimum duration for dehydration of zygotic embryos were standardized. The maximum retrieval of healthy plantlets was obtained from the embryos subjected to 18 hours silica gel or 24 hours laminar airflow desiccation pretreatment. Irreversible damage caused by desiccation to the death of the shoot meristem was noticed when the water content reduced to 20%.
DNA amplification fingerprinting in coconut: protocol optimization and analysis of resistance to root (wilt) disease K. Jayadev; M.K. Rajesh; K. Devakumar; Regi Jacob Thomas; R.V. Nair; V.A. Parthasarathy
International Coconut Community Journal Vol 21 No 1 (2005): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v21i01.396

Abstract

Root (wilt) disease is a serious malady in coconut causing a loss of approximately 968 million nuts a year in India. Integrated management practices are the only suggested methods to reduce its effect to some extent, while the development of resistant varieties will offer a permanent solution for which concrete breeding efforts are required. Considering the long life cycle of coconut, selection of resistant varieties through conventional methods will be time consuming and laborious. Molecular markers offer numerous advantages over markers traditionally used in plant mapping and selective breeding. Here, we present the optimization of PCR conditions for DNA amplification fingerprinting (DAF) of coconut using arbitrary oligonucleotide primers. Three of the primers could detect variations between root (wilt) resistant and susceptible coconut palms. This study constitutes the basis for future efforts to tag the root (wilt) resistant gene(s) in coconut.
COMBINING ABILITY IN COCONUT (Cocos nucifera) K.U.K.Nampoothiri; P.M. Kumaran; B.A. Jerard; M.J.Ratnambal E.V.V.B.Rao; V.A. Parthasarathy
International Coconut Community Journal Vol 15 No 02 (1999): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v15i02.330

Abstract

Combining ability has been proved to be a method to identify and select the parents and hybrids for increased production through the exploitation of heterosis. The exploitation of heterosis would be useful if suitable hybrids are produced for low yielding environmental conditions of littoral sandy soil. Combining ability effects for early germination, seedling girth, seedling height and breadth of laminate leaf on coconut have been reported by Louis and Chopra (1991) and on husked nut weight by Fernando (1996). Estimation of GCA and SCA variance will help to find out the gene action involved in a particular character and thus one can decide the breeding method for the improvement of the character. The present investigation was carried out to find out the general and specific combining ability effects of parents and hybrids at Central Plantation Crops Research Institute, Kasaragod.
Co-Authors Anitha Karun B.A. Jerard K. Devakumar K. Jayadev K. K. Sajini K. Muralidharan K.K. Sajini K.U.K.Nampoothiri M.J.Ratnambal E.V.V.B.Rao M.K. Rajesh P.M. Kumaran R.V. Nair Regi Jacob Thomas