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INVESTIGATION OF THE FEASIBILITY OF CONSTRUCTING A MAP FOR COCONUT WITH SEVERAL F2 FAMILIES USING COMPUTER-SIMULATED DATA C. K. Bandaranayake
International Coconut Community Journal Vol 19 No 01 (2003): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v19i01.370

Abstract

A computer simulation was performed using RiceSim computer software to explore the practicability of combining several different F2 populations together through JoinMap to mimic the real available coconut mapping populations, and found that it was very successful. JoinMap would be able to map all 16 chromosomes which covered the map length of 1540 cM except for a single marker on chromosome 8. The largest marker interval was 32 cM at the bottom of chromosome 3 and all other markers were evenly distributed along the chromosomes maintaining the space around 12-30 cM between them.
Happy mapping as an alternative to overcome the problems in coconut genome mapping C. K. Bandaranayake
International Coconut Community Journal Vol 20 No 2 (2004): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v20i02.389

Abstract

An excellent way of producing a reliable mapping population for quantitative trait loci analysis and marker assisted selection was considered. A physical mapping method known as ‘Happy Mapping’ was discussed to make a framework map as an alternative to overcome the problems associated with meiotic mapping.
DNA fingerprinting to distinguish the coconut type, San Ramon C. K. Bandaranayake; W. B. S. Fernando; A. Fernando; N. Herath
International Coconut Community Journal Vol 21 No 2 (2005): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v21i02.403

Abstract

San Ramon, an introduced type of coconut from Philippines, has long been in Sri Lanka. San Ramon has the advantage of producing high copra, high oil, and high-quality timber and also seemed to be less variable for bunch characters. In addition, it is a type of coconut having an ability to withstand long droughts and also coconut mites. Due to these outstanding beneficial characters, San Ramon has been used as a parent for effecting various crosses to transmit the drought tolerance nature and high copra outturn, to the offspring. The physical structure of San Ramon palms is much comparable to tall type coconuts though there are some specific characters predominantly seen in favourable environments. On top of these circumstances, it is not easy to separate San Ramon from other Talls and virtually impossible to distinguish pure San Ramon from San Ramon crosses by means of only morphological markers. Therefore, a molecular finger-printing method was attempted to distinguish pure San Ramon using already developed 10 coconut SSR primers. The results indicate the possibility of distinguishing pure San Ramon from its crosses with the help of 2 coconut SSR primers, CNZ6 and CNZ44.
An effective population size for reliable map resolution of coconut (Cocos nucifera L.) C. K. Bandaranayake
International Coconut Community Journal Vol 22 No 2 (2006): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v22i2.557

Abstract

Size of the mapping population is a critical landmark in producing a genome map of any crop. In coconut there is difficulty in producing a reasonably large mapping population within a specified period of time. Therefore revealing an effective population size for standard map resolution of coconut is needed. It is found that the size of a segregating population with 400 individuals is efficient for consistent map resolution of coconut.