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SUPRIADI, SUPRIADI
Balai Penelitian Tanaman Rempah dan Obat
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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EFFECTIVITY OF DRYING METHODS TO MINIMIZE AFLATOXIN OF DRIED NUTMEG Sembiring, Bagem BR.; Supriadi, Supriadi; Ediningsih, Ediningsih
Jurnal Penelitian Tanaman Industri Vol 26, No 1 (2020): June, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n1.2020.1-10

Abstract

Indonesian nutmeg export is constrained due to aflatoxin content that exceeds the minimum limit. This study was aimed at examining the effectiveness of three drying types of nutmeg to diminish aflatoxin contamination. The three drying types tested were (a) the rack type house with a source of solar energy heat combined with a kerosene stove fire, (b) the drying bases one meter above the ground with or without a black cloth cover, and (c) the floor drying 5 cm above the ground with or without black cloth cover. Unpeeled nutmegs were dried in the drying racks until they dried (sound when shaken by hand). After that they were. peeled and redried in the same type of dryer. Observation parameters included temperature, humidity, drying time, moisture content, oil content, oleoresin, myristicin, and aflatoxin content. The results showed that the drying time in the house-type drying rack was 51.5 hours, longer than the para-para and drying floor (30 hours). The temperature during drying in the drying house was 35.6-37.310C lower than the other two types of dryers. However, its humidity was the highest (40.71-49.33%). The moisture content of shelled nutmegs and peeled nutmegs from all drying types fulfill the requirements (< 10%). The total aflatoxin contamination in shelled nutmegs and peeled nutmegs was < 3.28 µg/kg, while B1 aflatoxin was < 1.06 µg/kg, so that they fulfilled SNI standard. All three drying types can be recommended to be tested further in field scale.Keywords: Nutmeg, Myristica fragrans, drying technology, seed quality.
PRODUKSI ENSIM KARBOKSI METIL SELULASE DAN EKSO-POLIGALAKTURONASE SERTA PERANANNYA DALAM MENENTUKAN TINGKAT PATOGENSITAS MULYA, KARDEN; HENI, HENI; SUPRIADI, SUPRIADI; ADHI, ESTHER M.
Jurnal Penelitian Tanaman Industri Vol 7, No 2 (2001): Juni,2001
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v7n2.2001.49-53

Abstract

Carboxy methyl selulase and exo-polygalacturonase enzymes production and their role in determining the pathogenicity of Ralstonia solanacearum isolated from gingerBacterial wilt disease on ginger (Zingiber oicinale Roscoe) caused by Ralstonia solanacearum is the most destructive disease Infected tissues show macerated symptom on infected hosts indicating that the pathogen produces plant digestive enzymes. This research was aimed at analizing carboxymethylccllulasc (CMC-ase) and exopolygalacturonase (cxo-PG) enzymes production by the pathogen. In vitro production of CMC-ase of both virulent and avirulcnt isolates of R solanacearum was measured from diameter of clearing zone around bacterial colony on CMC medium whereas exo-PG was measured by the reduction of Na- polygalacturonatc by filtrate of the pathogen culture. Virulence of the pathogen was tested on tomato cultivar Gondol Hijau by pouring 50 ml of pathogen suspension (I07 cfu/ml) around roots of the plant and it was also tested on ginger cultivar Jahe Putih Besar by pricking suspension of pathogen into rhizome and basal pscudostcm of the plant. The results showed that CMC-ase and and PG-asc were produced by virulent isolates of R. solanacearum al 2.23 cm clear zone and 0.662 mg eq. glucose/ml/hour/ODiti, respectively. The avirulent isolates, however. correlated with disease intensity of the isolates. Disease intensity of the virulent isolates was 0.6 and 0.96 on tomato and on ginger plants respectively, whereas the avirulent isolates was 0.04 and 0.00 respectively. Therefore, CMC-ase and exo-PG are important in determining pathogenicity level of/?, solanacearum.
EFFECTIVITY OF DRYING METHODS TO MINIMIZE AFLATOXIN OF DRIED NUTMEG Sembiring, Bagem BR.; Supriadi, Supriadi; Ediningsih, Ediningsih
Jurnal Penelitian Tanaman Industri Vol 26, No 1 (2020): June, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n1.2020.1-10

Abstract

Indonesian nutmeg export is constrained due to aflatoxin content that exceeds the minimum limit. This study was aimed at examining the effectiveness of three drying types of nutmeg to diminish aflatoxin contamination. The three drying types tested were (a) the rack type house with a source of solar energy heat combined with a kerosene stove fire, (b) the drying bases one meter above the ground with or without a black cloth cover, and (c) the floor drying 5 cm above the ground with or without black cloth cover. Unpeeled nutmegs were dried in the drying racks until they dried (sound when shaken by hand). After that they were. peeled and redried in the same type of dryer. Observation parameters included temperature, humidity, drying time, moisture content, oil content, oleoresin, myristicin, and aflatoxin content. The results showed that the drying time in the house-type drying rack was 51.5 hours, longer than the para-para and drying floor (30 hours). The temperature during drying in the drying house was 35.6-37.310C lower than the other two types of dryers. However, its humidity was the highest (40.71-49.33%). The moisture content of shelled nutmegs and peeled nutmegs from all drying types fulfill the requirements (< 10%). The total aflatoxin contamination in shelled nutmegs and peeled nutmegs was < 3.28 µg/kg, while B1 aflatoxin was < 1.06 µg/kg, so that they fulfilled SNI standard. All three drying types can be recommended to be tested further in field scale.Keywords: Nutmeg, Myristica fragrans, drying technology, seed quality.
PRODUKSI ENSIM KARBOKSI METIL SELULASE DAN EKSO-POLIGALAKTURONASE SERTA PERANANNYA DALAM MENENTUKAN TINGKAT PATOGENSITAS MULYA, KARDEN; HENI, HENI; SUPRIADI, SUPRIADI; ADHI, ESTHER M.
Jurnal Penelitian Tanaman Industri Vol 7, No 2 (2001): Juni,2001
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v7n2.2001.49-53

Abstract

Carboxy methyl selulase and exo-polygalacturonase enzymes production and their role in determining the pathogenicity of Ralstonia solanacearum isolated from gingerBacterial wilt disease on ginger (Zingiber oicinale Roscoe) caused by Ralstonia solanacearum is the most destructive disease Infected tissues show macerated symptom on infected hosts indicating that the pathogen produces plant digestive enzymes. This research was aimed at analizing carboxymethylccllulasc (CMC-ase) and exopolygalacturonase (cxo-PG) enzymes production by the pathogen. In vitro production of CMC-ase of both virulent and avirulcnt isolates of R solanacearum was measured from diameter of clearing zone around bacterial colony on CMC medium whereas exo-PG was measured by the reduction of Na- polygalacturonatc by filtrate of the pathogen culture. Virulence of the pathogen was tested on tomato cultivar Gondol Hijau by pouring 50 ml of pathogen suspension (I07 cfu/ml) around roots of the plant and it was also tested on ginger cultivar Jahe Putih Besar by pricking suspension of pathogen into rhizome and basal pscudostcm of the plant. The results showed that CMC-ase and and PG-asc were produced by virulent isolates of R. solanacearum al 2.23 cm clear zone and 0.662 mg eq. glucose/ml/hour/ODiti, respectively. The avirulent isolates, however. correlated with disease intensity of the isolates. Disease intensity of the virulent isolates was 0.6 and 0.96 on tomato and on ginger plants respectively, whereas the avirulent isolates was 0.04 and 0.00 respectively. Therefore, CMC-ase and exo-PG are important in determining pathogenicity level of/?, solanacearum.
Co-Authors ADHI, ESTHER M. Ediningsih, Ediningsih HENI, HENI MULYA, KARDEN Sembiring, Bagem BR.