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All Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Holistic: Journal of Tropical Agriculture Sciences
Revi, Lestia
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Agriculture, Biological Sciences & Forestry

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KARAKTERISASI MOLEKULER NUCLEAOPOLYHEDROVIRUS (NPV) HYPOSIDRA TALACA WLK. DI PERKEBUNAN TEH GUNUNG MAS BOGOR Kusumah, R. Yayi Munara; Revi, Lestia; Kurniawati, Fitrianingrum
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.771 KB) | DOI: 10.23960/j.hptt.217147-155

Abstract

Characterize molecular the Nucleopolyhedrovirus (NPV) Hyposidra talaca Wlk. of tea plantation at Gunung Mas Bogor. Hyposidra talaca is one of the most important pest in tea plantation, and generally attacks of leaves and shoots. This pest cause yield loss up to 40-100%. NPV can be pathogenic to the pest of H. talaca and can be developed as an alternative measure to control H. talaca in tea plantations and based management appears to be more ecofriendly and effective. However, information regarding characterisation molecular of HytaNPV is limited. The study conducted to characterize molecular the NPV of H. talaca by restriction nucleotide and amino acid, by using gens lef-8.  Molecular identification used Polymerase Chain Reaction (PCR) consisted of DNA extraction, DNA amplication, and DNA electrophoresis. DNA amplication using gen lef-8 showed positif result with approximately 770 bp. Gen lef-8 can identified HytaNPV. DNA sequance showed that isolate HytaNPV Bogor had high homology of pathogonic NPV of genus Helicoverpa from Brazil, Australia, Spanyol and Netherland with homology nucleotide and amino acid reached 98% and 100%. Based on philogeny tree of HytaNPV was one group with pathogenic NPV of genus Helicoverpa.
Barriers and readiness for implementation of Indonesian sustainable palm oil in independent smallhollders plantations: A case study Revi, Lestia
Holistic: Journal of Tropical Agriculture Sciences Vol. 2 No. 2: January (2025)
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/hjtas.v2i2.2025.1146

Abstract

Background: Smallholders have been required to implement a sustainable system in developing their plantations. Sustainability aspects that have been determined by the government that must be obeyed and fulfilled by every smallholder are listed in the principles and criteria of Indonesian Sustainable Palm Oil (ISPO). This research will focus on how smallhodelrs preferring and implementation gap ISPO certification requirements, as well as what factors cause these gaps. Methods: Data was collected based on direct interviews with 11 Village Unit Cooperative Administrators (KUD) in Sanggau Regency. The data was then processed and studied using the Gap Analysis method. Findings: The average implementation of ISPO in the respondent cooperatives is only 37 percent, which means that there is no implementation of ISPO in in the practice of cultivating oil palm plantations by independent smallholders. Independent smallholders need special training in its implementation some of the factors that cause gaps in the implementation of ISPO in independent smallholders in Sanggau Regency include organizations that are not well developed, limited access to finance. Conclusions:  The lack of information obtained by independent smallholders regarding the principles and procedures for implementing ISPO, as well as the lack of socialization of sustainable oil palm cultivation practices from both government and private institutions. One of solution to that problems is need a patner organization who can collaborate to implementation ISPO in smallholders. Novelty: This research contributes to the limited studies on ISPO implementation among independent smallholders, particularly in Sanggau Regency. Unlike previous studies that focus on large-scale palm oil industries, this study provides empirical insights into the practical challenges faced by smallholders.
Co-Authors Fitrianingrum Kurniawati, Fitrianingrum Kusumah, Yayi Munara