Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Shrestha, Bhupal Govinda
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Environmental Science

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Application of Atmospheric Pressure Argon Plasma Jet (APAPJ) in Biomedical Science and Engineering Gurung, Jyoti Prakash; Subedi, Deepak Prasad; Shrestha, Rajendra; Shrestha, Bhupal Govinda
Journal of Tropical Life Science Vol 10, No 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.931 KB) | DOI: 10.11594/jtls.10.02.07

Abstract

In this paper, we demonstrated the application of atmospheric pressure argon plasma jet (APAPJ) in biomedical science such as cancer therapy and biomedical engineering such as surface modification of polystyrene Petri dishes for animal cell culture. We observed that APAPJ, when exposed to breast cancer cell line (MDA-MB-231) for 60 seconds at a distance of ~ 2.5 cm, reduced the cancer cells by 57%. This reduction in cancer cell concentration demonstrated the cancer-killing property of APAPJ. Further optimization of APAPJ's various operational parameters and in-depth biochemical study is required to figure out the mechanism responsible for killing cancer cells. Also, we investigated cell adhesion and proliferation due to APAPJ treatment by Crystal Violet (CV) staining and MTT Assay. We observed that APAPJ treatment of polystyrene Petri dishes for 60 - 240 s at a distance of ~ 3.5 cm exhibited enhancement in adhesion of breast cancer cell line (MDA-MB-231). We also demonstrated the increase in hydrophilicity by a reduction in contact angle to 30.3°±3 after 240 seconds of APAPJ treatment of polystyrene samples. The hydrophilicity of the substrate has proven to enhance cell adhesion. Consequently, APAPJ could be employed as a simple, inexpensive and environmentally friendly method for surface modification of polystyrene plates for animal cell culture.
Study of Serum Lactate Dehydrogenase and Gamma-Glutamyl Transpeptidase in Breast Cancer Patients receiving Chemotherapy. Basnyat, Anil Singh; Jha, Abimanyu; Pathak, R; Shrestha, Bhupal Govinda
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.07

Abstract

Breast cancer (BC) is the most common type of cancer worldwide, being a major cause of morbidity in female. In Nepal it is the second most common type of cancer among females of perimenopausal age group. More than one quarter of the BC diagnosed in young Nepalese female, with familial history of breast cancer, early pregnancy, longer lactation and estrogen exposure and often with tumors showing aggressive biological behaviors. Anthracyclines (Doxorubicin) based treatment regime were reported to cause cardiotoxicity by increasing intramyocardial free radical production, lipid alterations and decreasing antioxidant level. Oxidative stress involving cellular reactive oxygen species (ROS) production is widely accepted mechanism but the molecular basis of chemotherapy induced organ toxicity remains highly controversial. An increased rate of metabolism and oxidative stress results rapid turnover of cancer cells that modulates the enzyme level in blood circulation. Serum LDH and GGT level correlates with tumor burden, metastatic character of BC and intensity of organtoxicity. The aim of our study is to evaluate the serum level of LDH and GGT in BC patients receiving chemotherapy and correlate these enzyme levels with different stages of BC. A total number of 150 subjects were included in the study, comprising 90 histopathologically confirmed 24 to 76 years aged patients of different breast cancer stages, receiving at least 3 cycles of 5-Fluorouracil, Adriamycin and Cyclophosphamide (FAC) chemotherapy. Sixty age- matched healthy women were enrolled as controls. Blood samples from each individual was collected after informed consent and analysed for serum LDH and GGT levels using standard biochemical methods. Data were analyzed using student's paired 't' test, Pearson correlation test and ANOVA. Serum LDH and GGT levels were significantly (p<0.001) increased in BC patients as compared to control group. When all 4 stages of BC were compared to control group, LDH and GGT showed steady and progressive increase in the activities of these enzymes from stage I-IV. The study concludes that serum LDH and GGT may be sensitive, specific and cost effective biomarkers in early diagnosis of breast cancer, assessing cancer prognosis and response to treatment.
Isolation and Screening of Potential Cellulolytic and Xylanolytic Bacteria from Soil Sample for Degradation of Lignocellulosic Biomass Shrestha, Bhupal Govinda; Ghimire, Sanjaya; Bhattarai, Shakep; Phuyal, Sailesh; Thapa, Bimal
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.06

Abstract

Cellulolytic/Xylanolytic microorganisms such as bacteria and fungi are accountable for conversion of lignocellulosic biomass in soil. Despite this vast number of cellulose/xylanase producers, there is a deficiency of microorganisms that can produce a significant amount of cellulase/xylanase enzyme to proficiently degrade cellulose/xylan to fermentable sugars. Although bacteria have extremely high natural diversity, which bestowsthem with the aptitude to produce stable enzymes, little emphasis has been given to cellulose/xylanase production from bacteria. Seven soil samples were collected from eastern hilly districts of Nepal viz. Taplejung, Panchthar and Sankhuwasabha districts, from soil surface and at depth of 10cm to 20cm, and were isolated separately. From the seven soil samples, four bacterial isolates were obtained. Isolates (PSS, P1D, TLC, SNK) were then screened for cellulolytic/xylanolytic activity using Congo red assay on Carboxymethylcellulose (CMC)/xylan agar plates. The enzyme activity obtained from isolates was dependent on substrate concentration. The activity of enzymes produced by isolates were also measured and compared on pretreated sugarcane bagasse. Among those samples, the greatest zone of inhibition in both CMC (1.3 cm) and xylan (1.0 cm) agar media was seen in isolate P1D. It also produced the highest activity of endoglucanase and xylanase i.e. activity 0.035 U/mL and 0.050 U/mL respectively at 0.010 mg mL-1 standard substrate concentration of CMC and xylan. 
Study of Phytochemical, Anti-Microbial, Anti-Oxidant, Phytotoxic, and Immunomodulatory Activity Properties of Bauhinia variegata Bhandari, Jaya; Thapa, Pratiksha; Niraula, Prasodhan; Thapa, Nita; Shrestha, Nikita; Shrestha, Bhupal Govinda
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.14

Abstract

There is growing interest in the use plants for the treatment and prevention of cancer and are currently being evaluated as promising anticancer agents. In this paper, we investigated the anti-microbial, anti-oxidant, phytotoxic and immunomodulatory of plant Bauhinia variegata and also established the presence of important phytoconstituents which might signify its anticancer property. Phytochemical screening, anti-microbial assay, phytotoxicity assay, anti-oxidant assay and immunomodulatory analysis was done according to the protocol established at Department of Biotechnology, Kathmandu University and Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Pakistan.In the phytochemical screening, we observed the presence of different phytochemicals like steroids, terpenoids, flavonoids, reducing sugars and glycosides. In case of Antimicrobial assay, the plant was found to be most effective against B.cereus with the highest zone of inhibition (ZOI) of 12mm and against Rhizopus with ZOI of 11mm. During the antioxidant assay in comparison to Ascorbic acid; at highest concentration, the scavenging activity shown by the plant Bauhinia variegata are 43.38% as compared to ascorbic acid 89.25%. In the Immunomodulatory assay at the highest concentration i.e. 250 µg/ml, the plant showed 75.1% effect, which showed that the plant has potential anti-inflammatory potential. In phytotoxicity assay, Bauhinia variegata showed 20% phytotoxicity. Bauhinia variegata has thus been proven to be an important candidate to be used as an antimicrobial, antioxidant, anti-inflammatory, phytotoxic and anticancer agent. Separation of pure compounds with bioassay guided extraction, spectrometric analysis and subsequent cytotoxicity assay of the pure bioactive compounds from Bauhinia variegate is highly recommended as crude extract itself showed promising phytotoxicity and other pharmaceutical potential.
Co-Authors Basnyat, Anil Singh Bhandari, Jaya Bhattarai, Shakep Ghimire, Sanjaya Gurung, Jyoti Prakash Jha, Abimanyu Niraula, Prasodhan Pathak, R Phuyal, Sailesh Shrestha, Nikita Shrestha, Rajendra Subedi, Deepak Prasad Thapa, Bimal Thapa, Nita Thapa, Pratiksha