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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Makara Journal of Science
Mohammadhassan, Reza
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Agriculture, Biological Sciences & Forestry Environmental Science

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A REVIEW OF PHARMACEUTICAL RECOMBINANT PROTEINS AND GENE TRANSFORMATION APPROACHES IN TRANSGENIC POULTRY Fallahi, Sepideh; Mohammadhassan, Reza
Journal of Tropical Life Science Vol 10, No 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.09

Abstract

Transgenic animals are employed to synthesize pharmaceutical recombinant proteins for three decades.  There are many problems to use farm mammalian animals for recombinant production such as high cost of production and maintenance, long generation interval, toxic effects of some human-source pharmaceutical proteins in other mammalian and incompatibility of human-source glycosylation with the other mammalian' glycosylation that all lead to low efficiency. Thus, transgenic poultry has been considered as the bioreactor of recombinant protein production. Increasing demand for pharmaceutical human proteins caused to make considerable efforts to develop transgenic poultry producing eggs contain recombinant protein. In the present review, at the first, transgenic animal and poultry are compared for their benefits and limitations. Then, the protein content of the egg, the features, gene and promoter of the egg are studied. After that, the recent achievements of the producing pharmaceutical recombinant proteins are considered. In the following, there are some explanations about gene transformation approaches in poultry, including sperm-, testis-, PGCs, and blastocyst-mediated methods depending on CRISPR/Cas9, Retroviral vectors, and DNA microinjection techniques, and embryonic manipulation approaches such as windowing and Ex ovo for introducing and injecting transformed cells into eggs.
Nanoelicitors Application Promote Antioxidant Capacity of Asparagus officinalis (In Vitro) Mohammadhassan, Reza; Ferdosi, Annahid; Seifalian, Alexander Marcus; Seifalian, Maral; Malmir, Shiva
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.01

Abstract

Nanoparticles recently play remarkable roles in modern agriculture and biotechnology due to specific exclusively physicochemical and biological characteristics of the particles. In recent years, nanoparticles are been using as fertilizers and elicitors to improve crops. Nutritionists are constantly seeking natural antioxidants without side effects to using for healthcare and treatment. Asparagus officinalis L. as medicinal plant treated by iron (0, 10, 50 and 100 mg/L) and selenium (0, 0.5,and 1 mg/L) nanoparticles as nano elicitors. Then the antioxidant capacity of A. officinalis L. was detected and measured by α, α-diphenyl-β-picrylhydrazyl (DPPH) assay, for assessment of the antioxidant activity. The iron nanoparticlesconcentration significantly increases the antioxidant activity of both male and female asparagus stem, as well as selenium nanoparticles. When combined iron and selenium used as nano elicitors then cause the antioxidant activity significantly decreases. But the integration of two nano elicitors (iron and selenium) decreased antioxidant capacity while the use of nano-selenium could enhance antioxidant capacity. The application of nano elicitor increased antioxidant capacity in the female stem than male.
Using In silico Tools to Analyze the 5ʹ Untranslated Regions of the Alcohol Dehydrogenase Gene from Arabidopsis thaliana and Omega Sequence Mohammadhassan, Reza; Asadishad, Tina
Makara Journal of Science Vol. 27, No. 4
Publisher : UI Scholars Hub

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Abstract

The 5ʹ ends of protein-encoding genes contain 5ʹ untranslated regions (5ʹUTRs), which can effectively participate in regulating gene expression. The 5ʹUTRs of Arabidospis thaliana–derived alcohol dehydrogenase gene (AtADH) and omega (Ω) sequence from tobacco mosaic virus (TMV) are the most effective enhancers in biotechnology. In this study, bioinformatics techniques were employed to analyze the characteristics of the above sequences. After 5ʹUTR sequence collection, the inner ribosome entrance sites; small RNA (sRNA) target sequences; nucleotide contents; and upstream start and stop codons, ORFs, and coding DNA sequences of the Ω sequence and AtADH 5ʹUTR were identified. Moreover, the free energies of secondary structures were calculated. Both 5ʹUTRs lacked upstream start codons and proteins, causing no interruptions in ribosome activity. The GC contents of the Ω sequence and AtADH 5ʹUTR were 24% and 30%, respectively. The Ω sequence contains more pyrimidines than AtADH 5ʹUTR. The Ω sequence included only three CAAT box regulatory elements. The free energy of the secondary structures of Ω was less than that of the AtADH 5ʹUTR. Two predicted secondary structures of Ω showed low complexity and free energy. Ω had a longer Inner ribosome entrance sites than the AtADH 5ʹUTR. In contrast to the AtADH 5ʹUTR, Ω was targeted by two sRNAs. Therefore, Ω is more powerful in enhancing ribosomal activity, translation, and protein expression than the AtADH 5ʹUTR.
Co-Authors Asadishad, Tina Fallahi, Sepideh Ferdosi, Annahid Malmir, Shiva Seifalian, Alexander Marcus Seifalian, Maral