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Purba, Andre Manusun
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Immunology & microbiology Social Sciences

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Sensitivity of Terminalia catappa L. Toward Aeromonas hydrophila Purba, Andre Manusun; Riauwaty, Morina; Syawal, Henni
Jurnal Perikanan dan Kelautan Vol 25, No 2 (2020)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.2.116-122

Abstract

Terminalia catappa is commonly present in tropical area  and its can be used for increasing pH in fish culture and it also be used as disinfectant.  A research aims to understand the dosages of T. catappa leaf extract to hamper the growth of Aeromonas hydrophila  has been conducted  from February  to July 2019.  A diffusion agar KIRBY BAUER method was applied to understand the sensitivity of T. catappa  toward the A. hydrophila. Treatments applied were P0 (Control, Oxytetracyclin (30 µg.Disk), P1 (100%), P2 (90%), P3 (80%), P4  (70%), P5 (60%), P6 (50%), P7 (40%), P8 (30%), P9 (20%), P10 (10%) P11 (9%), P12 (8%), P13  (7%), P14 (6%), P15 (5%), P16 (4%), P17 (3%), P18(2%), P19 (1%). Pure leaf extract was obtained from fresh leaf. The disk blank was wet with 50 L of leaf extract. A. hydrophila was cultured in TSA, TSB and GSP media, and it was grew up for 24 hours. Results shown that the pure leaf extract produce  13.9 mm diameter clear zone, while 2% of pure extract produce 6.5 mm diameter clear zone.
Sensitivity of Terminalia catappa L. Toward Aeromonas hydrophila Purba, Andre Manusun; Riauwaty, Morina; Syawal, Henni
Jurnal Perikanan dan Kelautan Vol. 25 No. 2 (2020): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.2.166-122

Abstract

Terminalia catappa is commonly present in tropical area and its can be used for increasing pH in fish culture and it also be used as disinfectant. The aimed of this study was to obtain a dose of T. catappa leaf solution which was able to inhibit the growth of Aeromonas hydrophila. This research was conducted from April to August 2019, at the Laboratory of Parasites and Fish Diseases, Department of Aquaculture, Faculty of Fisheries and Marine, Riau University. The method used in this study is an experimental method, namely disk diffusion KIRBY-BAUER to see the sensitivity of T. catappa leaf solution against A. hydrophila. The Kirby-Bauer method uses a 6 mm diameter blank disk, to reduce the level of error, then it is repeated three times. The treatments applied were K: Control (Oxytetracycline) at a dose of 30 µg / disk, P1: Giving T. catappa leaf solution 100% dose, P2 (90%), P3 (80%), P4 (70%), P5 (60%) , P6 (50%), P7 (40%), P8 (30%), P9 (20%), P10 (10%) P11 (9%), P12 (8%), P13 (7%), P14 (6%), P15 (5%), P16 (4%), P17 (3%), P18 (2%), P19 (1%). The results showed that the T. catappa leaf solution was able to inhibit the growth of A. hydrophila bacteria at doses of 100-2% with inhibitory zone diameters of 13.9-6.5 mm.
Co-Authors Henni Syawal