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POTENSI FUNGI ENDOFIT BIJI PINANG SEBAGAI ANTIBAKTERI TERHADAP ESCHERICHIA COLI DAN SALMONELLA THYPI Siska Nuryanti; Rusli; Risma Astuti
Green Medical Journal Vol 1 No 1 (2019): Green Medical Journal
Publisher : Fakultas Kedokteran Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.292 KB) | DOI: 10.33096/gmj.v1i1.23

Abstract

Fungi endofit adalah fungi yang terdapat dalam sistem jaringan tumbuhan, seperti daun, bunga, ranting ataupun akar tumbuhan. Fungi endofit memiliki kemampuan untuk memproduksi senyawa bioaktif, baik yang sama maupun tidak sama dengan inangnya tetapi seringkali memiliki aktivitas biologis yang serupa dengan senyawa bioaktif yang diproduksi inangnya Penelusuran fungi endofit dari biji pinang (Areca catechu L) sebagai pengasil senyawa antibiotika dilakukan dengan tujuan untuk memperoleh fungi endofit yang memiliki kemampuan untuk memproduksi senyawa bioaktif yang berkhasiat sebagai antibiotik. Metode pengujian aktivitas antibakteri yang digunakan pada penelitian ini adalah metode KLT-Bioautografi. Hasil isolat fungi endofit diperoleh enam isolat jamur (IFBP_I, IFBP_II, IFBP_III, IFBP_IV, IFBP_V), yang memiliki pencirian  makroskopik yang berbeda. Isolat mikroba murni hasil fermentasi diekstraksi dan di uji aktivitas antibiotikanya terhadap bakteri penyebab infeksi saluran pencernaan yaitu Escherechia coli dan Salmonella thypi. Ekstrak fermentat isolate fungi endofit biji pinang dapat menghambat bakteri Escherichia coli dengan nilai Rf 0,45, 0,65 dan 0,92, dan bakteri Salmonella thypi dengan nilai Rf 0,61 dan 0,85. Hasil identifikasi komponen kimia dari ekstrak fermentat isolate fungi endofit biji pinang yang menghambat pertumbuhan bakteri uji Escherichia coli dan Salmonella thypiadalah golongan senyawa alkaloid, tannin, dan polifenol
KARAKTERISTIK INDEKS MASSA TUBUH DAN JENIS KELAMIN PASIEN HIPERTENSI DI RS IBNU SINA MAKASSAR NOVEMBER 2018 rachmat faisal syamsu; siska nuryanti; muhammad yastrib semme
J-KESMAS: Jurnal Kesehatan Masyarakat Vol 7, No 2 (2021): J-KESMAS Volume 7, Nomor 2, Nopember 2021
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/jkesmas.v7i2.1903

Abstract

Hypertension, commonly referred to as high blood pressure, is a global health problem in Indonesia because of its high prevalence, although it varies in different countries. In Indonesia, hypertension is found in 83 per 1000 household members. The majority of high blood pressure increases with the presence of risk factors such as; Age, lack of exercise / physical activity, genetic factors and family history, body weight/obesity, sodium intake, alcohol consumption, smoking, stress. This study is a descriptive design using an observational approach to describe the characteristics of the risk factors for the Body Mass Index (BMI) and the gender of hypertension patients at Ibnu Sina Hospital for November 2018. The sampling technique used the total sampling technique with a complete sample size of 26 people. The results showed that the gender characteristics of hypertension sufferers showed that the number of female patients (16 people) was more than that of male patients (10 people). And the attributes of BMI in patients with hypertension found that the number of patients with normal nutritional status (13 people) was the most compared to another nutritional status.
Aktivitas Antibakteri Ekstrak Etanol Daun Ketepeng Cina (Cassia alata L.) Terhadap Beberapa Bakteri Penyebab Infeksi Kulit Ika Reski Fitriani; Fitriana Fitriana; Siska Nuryanti
Makassar Natural Product Journal (MNPJ) April 2023
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Skin infections are one of the health problems that often occur in tropical countries which can be caused by several microorganisms such as bacteria, parasites, viruses, and fungi. One of the plants that can be used to treat skin infections caused by bacteria is the Chinese ketepeng leaf (Cassia alata L.), which contains antibacterial compounds. The purpose of this study was to determine the antibacterial activity of the ethanolic extract of the leaves of Chinese ketepeng (Cassia alata L.) against several bacteria that cause skin infections. Chinese ketepeng leaf simplicia was extracted by maceration method and then evaporated to obtain a thick extract. Then an antibacterial screening test was carried out, testing the value of the Minimum Inhibitory Concentration (MIC), Minimum Kill Concentration (KBM) with variations in the concentration of each test of 0.5%, 0.1%, 0.2%, 0.4%, 0, 8%, 1.6%, 3.2%, 6.4%, and 12.8%, and the antibacterial activity test with varying concentrations of 5%, 10%, and 20% against several bacteria, namely Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Propionibacterium acnes. Based on the results, the MIC values ​​for Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa were 1.6%, and Propionibacterium acnes was 0.4%. The MBC value for Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes is 3.2%, and Pseudomonas aeruginosa is 6.4%. And the antibacterial activity test obtained the largest diameter of the inhibition zone at the optimum concentration of 20% on the test bacteria. Based on this research, the ethanolic extract of the leaves of the Chinese ketepeng (Cassia alata L.) has potential as an antibacterial.