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All Journal Jurnal AgroBiogen
Husin, Bahagiawati A.
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry

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Isolasi dan Purifikasi Inhibitor α-amilase dari Biji Kacang Phaseolus vulgaris Husin, Bahagiawati A.
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p7-12

Abstract

Plant genetic engineering technologies enable of introducing insect resistance genes into crop plants. The cry genes, genes encoding for inhibitor of digestive enzymes of a target insect that were isolated from Bacillus thuringiensis can be used for this purpose. Seeds of common bean (Phaseolus vulgaris) contain a glycoprotein that inhibits activity of α-amylase of insects. A α-amylase inhibitor was purified from the common bean seeds. The purified α-amylase inhibitor was then fed to cowpea storage weevil, Callosobruchus maculatus. The results showed a lengthened larval development time inside the seed and caused mortality to the insect larvae. This experiment suggests that the α-amylase inhibitor gene from the common bean seed could be used as a candidate gene for genetic engineering of plant resistance to bruchid insects.
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