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Roostika, Ika
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975
Agriculture, Biological Sciences & Forestry

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Perkembangan Aplikasi Teknik Kriopreservasi untuk Konservasi dan Mendukung Program Pemuliaan Tanaman Roostika, Ika
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p39-48

Abstract

In the beginning, cryopreservationtechnique was primarily used for germplasmslong-term storage as passive collection because cell divisionand metabolism process can be stopped at super lowtemperature, commonly in liquid nitrogen. The technique issuitable for vegetative propagated plants and recalcitrantseeds. Recently, its application is extending for storing manyspecies and orthodox seeds. In this paper, the developmentof cryopreservation application is discussed. In Indonesia,cryopreservation is being studied but the application ofcryopreservation has been significantly developed abroad.The application of cryopreservation technique is not only forpreserving passive collections but also for storing activecollections, including to provide plant materials for hybridization,cellular engineering, genetic transformation, as wellas pathogens eradication or cryotherapy. It is concluded thatcryopreservation plays an important role in conventional andmodern plant breeding program.
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