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All Journal An Idea Health Journal
Edwinanto, Ludovicus
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Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Ethanol Extract of Pomegranate Peel (Punica Granatum) in Inhibiting HeLa Cell Proliferation Nurfazriah, Latifah Rahman; Dhianawaty, Diah; Rezano, Andri; Edwinanto, Ludovicus
An Idea Health Journal Vol 4 No 01 (2024)
Publisher : PT.Mantaya Idea Batara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53690/ihj.v4i01.445

Abstract

Cervical cancer is one of the causes of death in women in Indonesia. Many studies have been conducted to determine the anti-cancer effects of natural ingredients. Pomegranate peel contains ellagic acid which has anti-cancer effects. In this study, the anti-cancer activity of pomegranate peel was examined against HeLa cell lines. Pomegranate peel was obtained from Malang, East Java, extracted using 70% ethanol. Cell viability and IC50 values were examined using the MTT test after incubation for 24, 48, and 72 hours. The percentage of living cells was calculated using a hemocytometer using Trypan Blue staining. DNA fragmentation was examined using electrophoresis as a marker of apoptosis activation. The IC50 value in pomegranate peel extract was at a concentration of 450 µg/ml. The results of the percentage of viability cells using hemocytomete at an IC50 concentration of 52%. Smears in DNA fragmentation examination were seen in samples incubated for 24 hours, indicating apoptosis activation. Pomegranate peel extract has anticancer activity against HeLa cell lines, with a mechanism involving apoptosis. This potential indicates that pomegranate peel extract can be further developed as a natural anticancer agent
Co-Authors Diah Dhianawaty Nurfazriah, Latifah Rahman Rezano, Andri