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Maftuch, Mr.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Environmental Science

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THE EFFECTIVITY OF RAW EXTRACT GUAVA LEAF IMMUNOSTIMULANT (Psidium guajava) ON LIVER HISTOPATHOLOGY OF PATIN (Pangasius sp.) TESTED THE CHALLENGE Aeromonas hydrophila BACTERIA Rahman, Ihsan Sanggar; Maftuch, Mr.; Sanoesi, Ellana
JFMR (Journal of Fisheries and Marine Research) Vol. 2 No. 2 (2018): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2018.002.02.1

Abstract

The purpose of this study described the influence and the dose of guava leaf raw extract (P. guajava) as an immunostimulant towards liver histotapologi Patin (Pangasius sp.) Challenge tested A. hydrophila bacteria. This study uses a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments used was of guava leaf extract doses A dose of 2% (2.4 g / 120 g), and 4% (4.8 g / 120 g), C 6% (7.2 g / 120 g) and K without a guava leaf extract and tested bacteria A. hydrophila challenge. Based on the results obtained in the study of liver histopathology Patin (Pangasius sp.), there was damage Inflammation and necrosis of the liver tissue in order from the most severe damage to the least obtained at treatment K (0%) 3,07, A ( 2%) 2.53, B (4%) of 2.33, and the best treatment is C (6%) with an average damage of 1.67. Necrosis of liver tissue damage in a sequence of severe damage to the least, namely the treatment of K (0%) 2,67, A (2%) 2.33, B (4%) 2.20, and C (6%) 1,53. Lowest average gained in treatment C (6%). The results showed that the dose can reduce the level of tissue damage that was at a dose of 6% (7.2 g / 120 g). The best dose is 6% (7.2 g / 120 g), which can reduce the level of damage indicate the level of tissue damage smaller percentage of damage 6-25%.
UJI DAYA HAMBAT EKSTRAK Chaetoceros calcitrans TERHADAP BAKTERI Aeromonas salmonicida Maftuch, Mr.; Setyawan, Febby Hadi; Suprastyani, Heny
JFMR (Journal of Fisheries and Marine Research) Vol. 2 No. 1 (2018): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2018.002.01.6

Abstract

The impact of use antibiotic can cause problems on aquaculture such as bacterial resistance and residues for consumer. Therefore, one of alternatives that can be used to inhibit or kill bacteria, one of which is the use of plankton Chaetoceros calcitrans. This research aims to know the influence of the granting C. calcitrans extract that can inhibit or kill bacteria and know the best dose for inhibit A. salmonicida bacteria. The experiment method  used randomize completely design consist of four treatment and two control. A treatment (0.3 ppm), B treatment (15.3 ppm), C treatment (30.3 ppm),  D treatment (45.3 ppm), positive control (add chlorampenicol 5ppm) and negative control (without extract). The result of research showed the highest average of clear zone diameter in D treatment 5.37 mm and the lowest average of clear zone diameter in A treatment 2.89 mm, with linear equation y = 3.2067 + 0.0509x and coefficients R2 = 0.9262. The relationship between C. calcitrans extract in inhibitng gwoth of A. salmonicida bacteria showed that the average diameter of the clear zone has increase in line with the addition of extracts. Form the research showed that the extract of C. calcitrans was bacteriostatic (inhibits the growth of bacteria) and best obtained at the treatment dosage of D with a dose of 45.3 ppm and included into the category of middle.
Co-Authors Heny Suprastyani, Heny Rahman, Ihsan Sanggar Sanoesi, Ellana Setyawan, Febby Hadi