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All Journal Jurnal Ilmu Kimia Universitas Brawijaya
Mahdi, Chanif
Brawijaya University
Chemical Engineering, Chemistry & Bioengineering Chemistry

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OPTIMASI AMOBILISASI UREASE DARI Schizzosaccharomyces pombe MENGGUNAKAN MATRIK Ca-ALGINAT Maharani, Laras Dwi; Prasetyawan, Sasangka; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Urease immobilization is required to be used in repetition The enzyme hydrolize urea to be ammonia and carbondioxide. Urease was isolated from Schizzosaccharomyces pombe and was purified through precipitation by using ammonium sulphate at 30-45% saturation level, followed by dialysis. Urease was immobilized by entrapment method in a matrix of Ca-alginate. The study aimed to determine optimum concentration of Na-alginate and enzym concentration. In this research, Na-alginate concentration (1,5 ; 2 ; 2,5 ; 3 ; 3,5) % (w/v) and variation of urease concentration (0,840; 1,680; 2,520; 3,360; 4,200) mg/mL. Protein content was determined by Biuret reagen and enzyme activity was determined as the amount of mikromol amonia per minute, and resulted amonia was analyzed by Nessler method. The results showed that the optimum condition of immobilization urease is achieved on 3% Na-alginate solution and 2,52 mg/ml urease yielding in 2,52 mg of entrapped enzyme/gram Ca-alginate and the activity of 0,55 Units. Key words: activity, Ca-alginate, Schizzosaccharomyces pombe, urease.
OPTIMASI AMOBILISASI UREASE DARI Schizzosaccharomyces pombe MENGGUNAKAN MATRIK KITOSAN-POLIETILEN GLIKOL Dewi, Hastini Artha; Prasetyawan, Sasangka; Mahdi, Chanif
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.708 KB)

Abstract

Schizosaccharomyces pombe is yeast that production urease and extraceluller enzyme. Urease can hydrolyze urea to be ammonia and carbondioxide. Free urease is unable to be reused, therefore urease is needed to immobilized in certain matrix. This research studies to determine optimum condition from chitosan concentration and enzyme concentration. Urease was immobilized by entrapment method using matrix chitosan-polyethylene glycol with make a variation to chitosan concentration (1.5; 2; 2.5; 3; 3.5) % (w/v) and make a variation to  urease concentration (0.840; 1.680; 2.520; 3.360; 4.200) mg/mL. Enzyme activity was defined as the amount of mikromol ammonia per minute, and resulted ammonia was analyzed by Nessler method. The results showed that the optimum condition of  immobilization uerase is achieved on chitosan concentration 3% (w/v) and urease concentration of  2.52 mg/mL and urease yielding in 2.36  mg/gram matrix chitosan-polyethylene glycol within the activity of  0.53 Units. Key words: activity, chitosan-polyethylene glycol, Schizosaccharomyces pombe, urease
Co-Authors Dewi, Hastini Artha Maharani, Laras Dwi Prasetyawan, Sasangka