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Formulasi dan Uji Aktivitas Antioksidan Sediaan Lip Balm dari Ekstrak Etanol Umbi Wortel (Daucus carota L.) dengan Metode DPPH (2,2-dipheny 1-picrylhydrazyl) Azima, Azima; Wahyuningsih, Sri; Agung, Yusfin Cesiana; Ilyas, Ika Lismayani
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 2 (2024): August 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i2.1145

Abstract

Umbi wortel (Daucus carota L.) mengandung banyak senyawa kimia salah satunya yaitu β-karoten. β-karoten berfungsi sebagai antioksidan alami. Penelitian ini dilakukan untuk mengetahui aktivitas antioksidan sediaan lip balm dari ekstrak etanol umbi wortel dengan metode DPPH. Pada penelitian ini, kestabilan sediaan diketahui melalui evaluasi sebelum dan sesudah cycling test meliputi uji organoleptik, uji homogenitas, uji pH, uji daya sebar, uji titik lebur dan uji kelembapan yang sesuai standar kestabilan untuk sediaan lip balm serta metode dalam uji aktivitas antioksidan sediaan lip balm ekstrak umbi wortel menggunakan metode DPPH. Hasil penelitian menunjukkan bahwa sediaan lip balm ekstrak umbi wortel stabil secara fisika dan kimia setelah melalui tahapan evaluasi sediaan dan menunjukkan hasil pada IC50 yang diperoleh berturut-turut pada formula 1 yaitu 29,18 ppm, formula II yaitu 20,18 ppm, dan formula III yaitu 10,47 ppm sedangkan pada kontrol positif lip balm viva memiliki nilai IC50 yaitu 3,60 ppm. Kesimpulan dari penelitian ini yaitu, sediaan lip balm ekstrak umbi wortel dikategorikan stabil secara fisika dan kimia serta mempunyai nilai IC50 yang paling baik pada formula III (6%) dengan nilai IC50 yaitu 10,47 ppm.Carrot tubers (Daucus carota L.) contain many chemical compounds, one of which is β-carotene. β-carotene functions as a natural antioxidant. This research was conducted to determine the antioxidant activity of lip balm preparations from the ethanol extract of carrot tubers using the DPPH method. In this study, the stability of the preparation was determined through evaluation before and after the cycling test, including the organoleptic test, homogeneity test, pH test, spreadability test, melting point test, and humidity test in accordance with stability standards for lip balm preparations as well as methods for testing the antioxidant activity of lip preparations. carrot tuber extract balm using the DPPH method. The research results showed that the carrot tuber extract lip balm preparation was physically and chemically stable after going through the preparation evaluation stages and showed the IC50 results obtained respectively in formula 1, namely 29.18 ppm, formula II, namely 20.18 ppm, and formula III, namely 10.47 ppm, while the positive control Viva lip balm has an IC50 value of 3.60 ppm. The conclusion of this research is that the carrot tuber extract lip balm preparation is categorized as physically and chemically stable and has the best IC50 value in formula III (6%) with an IC50 value of 10.47 ppm.
Perbandingan Metode Ekstraksi terhadap Kadar Flavonoid dan Aktivitas Antioksidan Ekstrak Etanol Daun Pulai (Alstonia scholaris) dengan Metode 2,2-difenil-1-pikrilhidrazil (DPPH) Wahyuningsih, Sri; Sari, Nur Indah; Wahid, Hilmiati; Azima, Azima
Sinteza Vol. 6 No. 1 (2026): February
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/sinteza.v6i1.31992

Abstract

Pulai leaves (Alstonia scholaris) are known to contain various secondary metabolites such as flavonoids, so the choice of extraction method is a factor that affects the quality and quantity of bioactive compounds produced. This study aims to compare the total flavonoid content and antioxidant activity of ethanol extracts of pulai leaves obtained through two different extraction methods, namely maceration and reflux. This study is a laboratory experimental study using 96% ethanol solvent in both extraction methods. The flavonoid content was determined quantitatively using UV-Vis spectrophotometry with quercetin as the standard. The antioxidant activity test was performed using the 2,2-diphenyl-1-picrylhydrazil (DPPH) method based on the IC50 value. The results showed that the extract obtained using the reflux method had a higher flavonoid content of 17.11±0.03 mgQE/g and an IC50 value of 7.84±0.02 ppm, while the maceration method produced a flavonoid content of 8.42±0.02 mgQE/g and an IC50 value of 12.53±0.17 ppm. As a positive control, vitamin C had an IC50 value of 5.26±0.08 ppm. Based on these results, the extraction of pulai leaves (Alstonia scholaris) using the reflux method proved to be more effective in extracting flavonoid compounds and had higher antioxidant activity compared to the maceration method.