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All Journal Indian Journal of Forensic Medicine & Toxicology
Nada S. Salah1, Thamer A.A. Muhsen1, Mohssen H. Risan2
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Antifungal Activity of Silver Nanoparticles Using Penicillium Chrysogenum Extract Against The Formation of Biofilm for Candida Glabrata Nada S. Salah1, Thamer A.A. Muhsen1, Mohssen H. Risan2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2820

Abstract

The results showed that 71 isolates of Candida spp were isolated from patients with leukemia both women and men, Isolate 59 from C. glabrata while the number of isolates of C.albicans, C.tropicalis, C.krusei and C.kefyer were 6,3,2,1 respectively. The size of the nanoparticle was measured using AFM, The highest peak was 455 nm due to the presence of surface plasmons and another 243 nm wavelength, SEM showed the presence of particle of different sizes and distributed regularly and small silver nanoparticle. Effect of synergistic silver nanoparticle and antifungal agent (fuconazole) on the biofilm of Candida glabrata, capable of C.gabrata on adhesion of epithelial cells in the absence of silver nanoparticle and fluconazole, no adhesion between epithelial and yeast cells when adding silver nanoparticles, Decrease in surface adhesion between biofilm of the yeast and the epithelial cell when adding fuconazole, When collecting silver nanoparticles with fluconazole and adding it to epithelial cells exposed to C.glabrata, It led to the inability of the yeast to adhere to epithelial cells and then died . All experiments showed the least significant differences at 0.001 level.
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