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All Journal Indian Journal of Forensic Medicine & Toxicology
Nawras Abdul Bari Madlol 1, Qassim Jawad Ameer 2
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Public Health

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Multiplex PCR for detection of the predominant diarrheacausing protozoa (Giardia intestinalis, Entamoeba histolytica, and Cryptosporidium parvum) in fecal samples of cattle from Babylon Province, Iraq Nawras Abdul Bari Madlol 1, Qassim Jawad Ameer 2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.3000

Abstract

The work, here, was focused on the detection of the predominant diarrhea-causing protozoa (Giardia intestinalis, Entamoeba histolytica, and Cryptosporidium parvum) in fecal samples of cattle from Babylon province, Iraq. Fifty fecal samples from 50 cattle were collected and processed using a specific multiplex polymerase chain reaction (mPCR) technique that employed the small subunit ribosomal RNA (SSrRNA) gene as a molecular target. Using specific primers for each protozoan, the results showed positive identification of the E. histolytica, G. intestinalis, and C. parvum in 38 (76%), 22 (44%), and 12 (24%), respectively, of the fecal samples. The data from the current investigation identify the presence of the E. histolytica, G. intestinalis, and C. parvum in the fecal samples collected from cattle located in Babylon province, Iraq.
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