<![CDATA[Background: An ideal method for gender identification would be accurate, simple, and cheap,enabling its use in most laboratories. In addition, it should also be able as much as possible to be used for all animals as well as tissues and/or cells. For chromosomal analysis, we need viable cells that are able to divide, and if this is not possible, these methods cannot be used. On the other hand, genetic methods are reliable and do not need living cells, and it is easy to obtain DNA for these studies even in very ancient and nonviable tissues. These methods are therefore the most accepted ones The current study aimed to Determine the gender of a given DNA samples by designed vaulable specific-PCR tool. Methodology: Thirty eight ( ninteen from each male and female) human blood samples were collected using EDTA-Na2 tubes for direct DNA extraction after taken the agreement of the volunteers to give the blood sample . via expert .The blood sample mixed well by rotation of EDTA tube. two sets of species specific primer pairs targeting Homo sapiens (AMLE X and AMLE Y) genes. were designed and checked. PCR and sequencing were performed and sequences were analyzed and register in GenBank. Results: the results revealed that, the amplicon of AMLE X gene of Homo sapiens were 1164bp and AMLE Y were 744bp Conclusion: The current study conclude that, validity and accuracy of designed 12S rRNA species specific primer pairs for human and nonhuman animal blood typing as a forensic tool and there is no intraspecies cross amplification.]]>
