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Sanaa Ghali Jabur1 , Murtada Hasan Abed2
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Genetic Survey of Enteroaggregative E.coli in Diarrheic Children under 5 years in Thi-qar governorate Sanaa Ghali Jabur1 , Murtada Hasan Abed2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10609

Abstract

The study aimed to investigate the prevalence of Enteroaggregative E.coli obtained from children of Thi-qar suffered from diarrhea through PCR detection of virulence factors (aggR, astA, pic) antibiotic resistance and extended spectrum ?-lactamase (ESBL) production. The investigation included collection 430 stool samples from diarrheic children under five years involved both gender and 40 samples as a control from healthy children , were consulted in (Bint al- Huda Hospital for Gynecology obstetrics and pediatrics , Al-Mousawi hospital and private clinics laboratory ) at a period extended from 10th December 2018 -21th July 2019. A total of the isolates were diagnosed by different laboratory and molecular method. PCR diagnosis confirmed the distribution of genes (aggR and astA) within EAEC were (66% and 53%), respectively. The highest resistance to antibiotics was (100%) to ceftazidime and cefpodoxime , (97% ) to ceftriaxone, followed by (96%) for Ampicillin, Sulfa-Trimethoprim and cefuroxime, respectively, and cefixime, amoxiclav, Cefotaxim and Tetracycline (94.8%), (92.3%), (85%) and (78.2%), respectively. While the medium resistance was (55.13%) for each of ciprofloxacin , piperacillin-tazobactam, respectively, and Amikacin (51%). While the lowest resistance was to Gentamicin (30.7%) and Imipenem (26.9%). EAEC isolates were multidrug resistance in average 64/66 (96.97%).
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