Objective: To evaluate the effectiveness of the educational program on nurses-midwives’ preventivemeasures about puerperal sepsis.Method: quasi-experimental study design carried among 60 Nurses and Midwives chosen by non-probabilitysample (purposive sample) at maternity hospitals. The data collected by the observation checklist Pretestand posttest after the implementation of the educational program. Data were analyzed using descriptivestatistical data analysis approach of frequency, percentage, mean, mean of scores, a total of scores, rangeand standard deviation and inferential statistical data analysis approach T-test, multiple linear regressions,person correlation coefficient, and analysis of variance (ANOVA).Results: (35.3%) of them at age 20-29 years old, (75%) of them had secondary nursing school graduate levelof education and (35%) of them had 11-15 years of services in the nursing field. there is a highly significantdifference at P < 0.01 between the initial period of Pretest and posttest of the study sample preventivemeasures toward puerperal sepsis. The grand mean at pretest was (1.69) and at posttest (2.38). There is astatistically significant difference between years of service and preventive measures at posttest and Pretestof the educational program at (p-value > 0.05).Conclusion: there is an improvement in the nurses-midwives’ preventive measures level at the periods oftests after the implementation of the educational program.