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All Journal Indian Journal of Forensic Medicine & Toxicology
Qusay Ahmed Olaij1 , Abdul Majeed Abdul Aziz Humadi2
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Detection of the Genetic Diversity and the DNA of Some Species of the Cruciferous Family (Brassicaceae) using the RAPD-PCR Markers Qusay Ahmed Olaij1 , Abdul Majeed Abdul Aziz Humadi2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11761

Abstract

The current study aimed to uncover the genetic diversity of eight local species of the Brassicaceae plantsby using RAPD markers, where the genetic relationship was determined, the genetic dimension was foundbetween the studied species and the genetic finger print of some of these species was identified. (DNA)was extracted from the young leaves of the cruciferous family plants using the method Ready (Kit). Theconcentration of the extracted DNA ranged from 148.35-165.3 ng /µl with a purity of 1.53- 1.82. The resultsobtained from the study showed that 307 band were all differentiated (100%), higher in number than bands(38) with (12.38%) in the primer (MQ10), lower in number than bands (21) and with (6.84%) in the primer(MQ6). The highest molecular volume (pb2500) was characterized by (B1) in the primer (MQ7), while theless Molecular volume (100bp) in the two species (B3 and B5) was distinguished by the primer (MQ8). Theresults of the genetic dimension showed that the lowest value is for the genetic dimension (0.260) appearedbetween the two species (B2 and B3), and the highest value of the genetic dimension was (0.975) appearedbetween the two types (B4 and B5). The phylogenetic tree was drawn between the studied species and thespecies were divided into two major groups. The first group included the species (B1, B2, B3 and B4) andthe second group included the species (B5, B6, B7 and B8).
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