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All Journal Indian Journal of Forensic Medicine & Toxicology
Ali Hassoun Hammadi
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Phenotype Detection of Genetic Enzymes B- Lactamase Isolation of Patients with Urinary Tract Infections Bacteria Escherichia Coli Ali Hassoun Hammadi
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11803

Abstract

Background: the main cause of multidrug resistant bacteria is the production of ?-lactamase enzymes. Theobjective of this study was to determine the prevalence of genotype of Escherichia coli spp. isolated fromnosocomial urinary tract infections (UTI). Methods: In a cross-sectional study from May 2019 to January2020, mid-stream urine specimens were collected from 210 patients presented with acute urinary tractinfections whom attending Al- Shahid Ghazi and Al-Hariri Hospital in Medical City/ Baghdad. Sampleswere cultured for E. coli isolation. Antibiotic sensitivity test and the prevalence of resistance enzymeproducing gen were investigated. Results: A total of 210 E. coli were isolated, multidrug resistance strainof E coli (92.85%) was reported by this study. PCR technique indicated that 88.57% of ESBL-producingisolates possessing blaTEM, 30% blaCTX-M genes and 20.48% blaSHV genes between isolates and E. colistill the less resistant to carbapenem due to low expression of KPC gen. Conclusion: ESBLs and KPC werethe higher prevalent genotype of multidrug resistant E. coli in urine sample.
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