<![CDATA[A total 248 clinical samples of cerebrospinal fluid (CSF) were collected from meningitis patients, for theperiod of July to October 2018 from the Child Protection Teaching Hospital in the Medical City Compoundin Baghdad. All isolates were identification depending on macroscopic, microscopic, biochemical testsand definite with Vitek-2 compact system. The results showed a growth in 42 samples. Sixteen isolates ofStaphylococcus spp. were obtained from samples. The number (percentage) of isolates according to speciesas follow: 7/16) 44%) of S. epidermidis, 3/16 (19%) of S. hominis, 3/16) 19%) of S. haemolyticus, 2/16(12%) of S. aureus, 1/16 (6%) of S.warneri. These isolates were tested for ability to biofilm formation byusing Luria Broth and Tryptone Soya Broth (TSB) supplemented with 0.25% and 2% glucose. The abilityof the isolates to form biofilm with Luria Broth, for S.epidermidis, S. aureus, S. haemolyticus, S.hominis andS. warneri were 7/7 (100%), 1/2 (50%), 2/3 (66.66%), 1/3 (33.33%),1/1 (100%) respectively not formingbiofilm.As for using a TSB supplemented with 0.25% glucose, S.epidermidis, S. aureus, S. haemolyticusand S.hominis were 6/7 (85.71%), 2/2 (100%), 2/3 (66.66%) and 3/3 (100%) respectively were weaklybiofilm formed and when using TSB supplemented with 2% glucose, showed that all Staphylococcus spp.(100%) were positive for the test..The study showed the effect of minimum inhibition concentration(8 ?g/mL) and minimum bactericidal concentrationand (16?g/mL) of Erythromycin on biofilm of Staphylococcusspp. Staphylococcus spp. isolated from CSF showed weak ability to biofilm formation and depending on thehost content of sugars . This study showed also weak biofilm forming of Staphylococcus spp. was resistenceto erythromosin.]]>
