Samar Ahmed Darweesh1 , Hamsa Muneam Yaseen2 , Ruaa Muayad Mahmood3 , Rokayia Samir Al-Khalisy4 , Husam Saleem Khalaf5
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Spectrophotometric Determination of Ampicillin with Sulfanilic Acid by Oxidative Coupling Reaction Samar Ahmed Darweesh1 , Hamsa Muneam Yaseen2 , Ruaa Muayad Mahmood3 , Rokayia Samir Al-Khalisy4 , Hu
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11844

Abstract

Sensitive, economic, simple and accurate spectrophotometric method for estimation of ampicillin in bulkand dosage form is described. Ampicillin was oxidized by sodium hypochlorite and coupling with sulfanilicacid in the presence of sodium hydroxide to obtain a stable yellow colored chromogen which exhibit amaximum absorption (?max) at 400 nm. The optimum conditions were carefully evaluated .Plot of absorbance against concentration was linear over the range (50-300 µg.ml-1). This method was applied tothe estimation of ampicillin in pure drug and commercial formulations successfully.