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All Journal Indian Journal of Forensic Medicine & Toxicology
Morteta H. Al-Medhtiy1,2, Hutheyfa Al-Salih1 , Hazem Almhanna1 , Ahmed H. Al-Dabhawi1
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Investigation Study of Human Spermatozoa Proteome Morteta H. Al-Medhtiy1,2, Hutheyfa Al-Salih1 , Hazem Almhanna1 , Ahmed H. Al-Dabhawi1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11877

Abstract

The current study has been conducted on health human sperm to identify the different types of amino acidsand entire proteins of sperm. Two methods have been applied to characterise of human sperm, firstly extractlysate of sperm, then digest and run it in mass spectrometry, secondly, total sperm lysate was run in SDSpage gel and bands were stained with coomassie, then band was cut and digest to extract of whole aminoacids and also was run in mass spectrometry. Result revealed that sperm have several proteins, which wereinvolved with different binding proteins, soluble proteins, and glycoproteins, transmembrane proteins.Current result was displayed that mass spectrometry can be used to recognise data of proteins of spermand detected types of it. This technique was applied on whole lysate and gel bands of sperm and createddatabase of sperm proteins which can be investigated and consider more interesting for researchers. Thisstudy is concluded that human sperm have important transmembrane, enzymes, glycosylated proteins,glycoproteins and uncharacterised proteins which might be answered many theoretical aspects of biologicaland biochemical sperm migrations before and after fertilization.
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