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All Journal Indian Journal of Forensic Medicine & Toxicology
Deepti Shrivastava
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Public Health

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Study of Fresh Versus Frozen Embryo Transfer with Reference to Early Pregnancy Loss Deepti Shrivastava
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12723

Abstract

Background: Etiology of Increased rate of early abortions associated with pregnancies happened afterIn Vitro Fertilisation are not understood fully till date. ‘Freeze all embryos’ is a new dictum of improvingthe overall success of In Vitro Fertilisation,although it’s effect on early pregnancy loss is controversial inexisting literature .So many studies stated that vitrification and thawing process although improves thepositive pregnancy rate but cause early pregnancy losses after implantion, but other studies contradict it.Hence in present study we wish to find out the difference of early pregnancy demise in frozen thaw embryotransfer cycles in comparison to fresh embryo transfer cycle.Trial Design: This is a cross sectional study which will be conducted in Wardha test tube baby centre,Acharya Vinoba Bhave Rural Hospital.Method: 20 consecutive women conceived after In Vitro Fertilisation, either Fresh or Frozen embryotransfer and will be followed up further for rate of early abortions amongst them.Expected Outcomes: Frozen embryo transfer cycles may have more chances of successful clinicalpregnancies at 6-7 weeks of gestational age, but later on may result in early pregnancy losses.
Study of Sperm Dna Fragmentation Index in Patients from Rural Areas of Wardha Region Kanchi S. Goenka; Akash More; Mehul Salve; Deepti Shrivastava
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14300

Abstract

Background: Sperm DNA fragmentation test is an advanced test for proper sperm function with respectto semen analysis, as semen analysis is used for motility and morphology assessment, it cannot explainsperm at molecular level and thus SDF test has been introduced to evaluate male fertility. The integrity ofgenetic material in sperm is crucial for successful fertilization & normal embryo development as this willlead to proper transmission of genetic information. Patients having varicocele, recurrent failure of ARTtecniques and improper lifestyle (radiation, smoking, alcohol, BMI, ageing, etc) are considered for spermDNA fragmentation test. SDF test is useful in selecting patients for the ART procedures i.e. IVF/ICSI/IUIand highest pregnancy and live birth outcomes.Objectives:· To predict male infertility with normal semen parameters & its diagnostic values.· To investigate the clinical factors related to sperm DNA fragmentation in male with infertility.· To investigate the rate of sperm DNA fragmentation in male related to improper fertilization, embryodevelopment and recurrent pregnancy loss.Methods: The participants (n=40) with male fertility problems will be included in randomised control trial.All 40 participants will be randomly selected to evaluate their sperm DNA integrity from their respectivesemen samples using sperm chromatin dispersion test.Discussion: Efficiency of the Sperm DNA Fragmentation test is assessed by performing sperm chromatindispersion test on respective processed semen sample using Halosperm G2 test kit.
Co-Authors Akash More Kanchi S. Goenka Mehul Salve