Introduction: Planned nursing intervention and health education regarding exclusive breastfeeding (EBF)improved knowledge, practice and attitude of mothers and contributed to prevention of EBF discontinuationwhich can consequently reduce the worldwide infants’ morbidity and mortality rates. Aim of theStudy: The present study aimed to investigate the effect of nursing intervention on prevention of EBFdiscontinuation. Study Design: A quasi-experimental design was utilized. Setting: Obstetric OutpatientClinic, Breast Feeding Room in NICU of Children’s Hospital affiliated to Ain Shams University hospitalsand MCH center in Abassia sector/Cairo/Egypt. Subject: A purposive sample of 100 mothers of infantsaged from birth up to 6 months. Tools of the Study: Predesigned questionnaire to assess: mothers andinfants characteristics, knowledge regarding factors affecting EBF discontinuation and its prevention,Iowa Infant Feeding Attitude Scale and observational checklists to assess mothers? breastfeeding practice.Results: It was clear that the majority of the studied mothers had good knowledge, positive attitude towardsprevention of EBF discontinuation and improved practice of breastfeeding post implementation of nursingintervention. There was statistical significant difference between total knowledge of the studied mothersregarding factors affecting EBF discontinuation and its preventionand total practice of breastfeeding pre/post nursing intervention. Conclusion: Nursing intervention had a positive effect on prevention of EBFdiscontinuation. Recommendation: Developing initiatives,pre and postnatal health education programs forprotecting, promoting and supporting breastfeeding which aid in the prevention of EBF discontinuation.