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All Journal Indian Journal of Forensic Medicine & Toxicology
YasameenHasan Ali1 , Tariq M Quasim2 , DBEL Wahab R.Alshaikly3
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Phylogenetic Analysis of TTV (TorqueTeno Virus) in Iraqi Woman Suffering of Failure Kidney Disease YasameenHasan Ali1 , Tariq M Quasim2 , DBEL Wahab R.Alshaikly3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13648

Abstract

There are still very few studies of the Torque Tenovirus infection rate between hemodialysis patients inIraq. Thus, the aim of this study was to assess the frequency of TTV viremia in hemodialysis Iraqi patients.This study has carried out in Baghdad city from October 2019 to February 2020 and included 150 patientswith kidney failure disease undergoing hemodialysis and 50 control subjects .Blood samples have beenobtained for serological TTV detection. The result shows PCR has been used to detect TTV, isolates havebeen identified through N22 region sequencing and phylogenetic analysis has also been performed. Of the150 patients, 2 (1 male and 1 female) were TTV positive.The results shows in ( isolate 14) substitutionfour Transversion A>C, C>G,A>T, and T>A three Transition T>C,A>G, and T>C of Specific region withinTTV(N-22) and showed 94% identified with a standard in Gene Bank while having 99% identified witha standard in Gene Bank with (isolate 17) substitution fiveTransversion A>T,A>C,T>A, A>Tand C>GthreeTransition A>G,A>G and T>C . The phylogenetic analysis showed that identical among themselvesand the world with 100% compatibility values.
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