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All Journal Indian Journal of Forensic Medicine & Toxicology
Noor Mohammad Ali1 , Aamal Ghazi Mahdi Al-Saadi1
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Public Health

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Molecular, Biochemical, and Phenotypic Identification of Phenol and Cresol Degrading Acinetobacter baumanni Strain Selected from Al-Rumaila Oil Well Noor Mohammad Ali1 , Aamal Ghazi Mahdi Al-Saadi1
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13773

Abstract

Background: phenol and cresol are dangerous compounds that have harmful effects on aquatic life, plantsand, many other organisms. Therefore, it is necessary to effectively eliminate these compounds to protectthe environment.Aim: The aim of this study is to select and characterizebacterial strain(s) from oil contaminated soils andtesting its ability for biodegrading phenol and cresol.Methods: Biochemical tests were performed in addition to morphological and molecular detection through16S rRNA analysis to identify the selected strain after growing on Mineral salts medium (MM) containingphenol or cresol . The residual concentration of phenol and cresol was analyzed over the time period bymeans of the 4-aminoantipyrine assay .Results: the selected strain was identified as Acinetobacter baumannii strain HILLA-1, and it was able toremove 100% of the phenol at all the used concentrationsafter 48 h. This strain was also able to degrade 100%of cresol at concentrations (25,50,75,100,200 ppm /L) and99.37%,88.69%,and 94.73% at concentrations(300,400,500 ppm /L) respectivelyafter 48 h.Conclusion: The selected and identified Acinetobacter baumannii strain HILLA-1 can provide a putativesource for bioremediation of phenol and cresol contaminated environments.
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