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All Journal The Journal of Pure and Applied Chemistry Research Jurnal Kimia Riset Warta AKAB
Afriani, Kartini
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Warta AKAB

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Pembuatan dan Pengujian Efektifitas Antibakteri Gel Pembersih Tangan Berbahan Aktif Kitosan Afriani, Kartini; Ismail, Ismail; Agustin, Puspita Ade
WARTA AKAB Vol 45, No 1 (2021): Warta AKAB
Publisher : Politeknik AKA Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.304 KB) | DOI: 10.55075/.v45i1.4

Abstract

Kitosan dapat dimanfaatkan sebagai bahan aktif dalam gel pembersih tangan. Kitosan memiliki sifat antibakteri terhadap bakteri Gram positif dan negatif. Gel pembersih tangan dibuat dengan variasi penambahan ekstrak kitosan sebesar 0%, 1%, dan 1,5%. Efektifitas antibakteri gel pembersih tangan kitosan dievaluasi menggunakan metode replika. Masing-masing gel pembersih tangan dibandingkan dengan kontrol positif melalui jumlah koloni bakteri yang terbentuk.Jumlah koloni bakteri yang terbentuk pada formula 1, 2, dan 3 berturut-turut sebesar 4, 2, dan 0, sedangkan koloni yang terbentuk pada kontrol positif sebesar 5. Analisis statistika menggunakan ANOVA onewaymenunjukkan gel pembersih tangan dengan konsentrasi ekstrak kitosan 1,5% (Formula 3) lebih efektif dalam menurunkan jumlah koloni bakteri pada tangan.
Validasi Metode Penetapan Bahan Aktif Permethrin dalam Insektisida dengan High Performance Liquid Chromatography (HPLC) Walansyah, Hasbi Bagja; Afriani, Kartini; Salim, Agus; Kholidanata, Fareka
Journal Warta AKAB Vol 47, No 1 (2023): Warta AKAB
Publisher : Politeknik AKA Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55075/wa.v47i1.167

Abstract

Insecticides are substances or chemicals that are used to kill or eradicate insects. One of the active ingredients contained in insecticide products is permethrin. One of the tests carried out was testing the levels of the active ingredient such as permethrin in insecticides. This testing was carried out by High Performance Liquid Chromatography (HPLC). This method is a new method that will be used for routine analysis testing laboratory at Laboratory. The method that is used refers to the journal Validated High Performance Liquid Chromatography (HPLC) Method for Quantifying Permethrin in Pharmaceutical Formulations. Therefore, it is necessary to validate this method regarding elements that can cause variations in the test results and identify factors that can affect the performance of the method and determine the magnitude of the effect. Based on the test results, it can be concluded that all the validation parameters of the testing method for determining the active ingredient permethrin by high performance liquid chromatography gave the results of specificity, linearity, instrument detection limit (LDI), quantitation limit (LQ), repeatability precision, intermediate precision, accuracy, and the rigidity of the method meets the CIPAC 3807 requirements. Keywords: Insecticide, Permethrin, CIPAC 3807 Keywords: Insecticide, Permethrin, CIPAC 3807
Co-Authors AA Sudharmawan, AA Agustin, Puspita Ade Ismail Ismail Kholidanata, Fareka Muhammad Ridwan Puspita Ade Agustin Tri Sutanti Budikania Vania Dwi Wardani Walansyah, Hasbi Bagja