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All Journal Indonesian Journal of Tropical and Infectious Disease JURNAL ANALIS LABORATORIUM MEDIK
Onesiforus, Benaya Yamin
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Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Correlation of Nutritional Status with Hookworm and Strongyloides stercoralis Infection in Children Under Five Years in Kokar Public Health Center, Alor Regency, East Nusa Tenggara Onesiforus, Benaya Yamin; Lalangpuling, Indra E.; Wijayanti, Mahardika A.; Murhandarwati, E. Elsa Herdiana
Indonesian Journal of Tropical and Infectious Disease Vol. 8 No. 3 (2020)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v8i3.12570

Abstract

Malnutrition can reduce immune response particularly in cytokine (IL-4, IL-5, IL-10) production and immune effector (eosinophil, IgE, and mast cell), thus increasing the probability of intestinal nematode infection. Through this study, intestinal nematode infections occurred among children under five years, at different nutrition status, in Kokar Public Health center, Alor Regency, East Nusa Tenggara was captured. Hookworm and Strongyloides stercoralis were studied as both of them have devastating impacts compare to other helminthes compare to other helminths. This study is a cross- sectional study with a quote sampling technique. As many as 238 children, aged 12-59 months living in Kokar's Public Health Center area, Alor regency were recruited in this study i.e. 7.7% severely underweight, 19.2% underweight, 70.5% normal and 2.6% overweight. Data were collected in August - October 2016. Hookworm and S. stercoralis infection were determined from collected fecal samples of all subjects using either Baermann test, Koga Agar Plate (KAP), or Harada- Mori culture method. The prevalence of hookworm and S. stercoralis infection was 8.82%, and 0,42%. Correlation between nutritional status and hookworm infection were analyzed by Mann-Whitney test with p value = 0.54 (p > 0.05). Prevalence of hookworm and S. stercoralis among children under five years in Kokar were 8.82% and 0.42%. There was no significant correlation between nutritional status with hookworm infection prevalence.
PERBANDINGAN METODE DEHIDRASI TERHADAP KUALITAS PREPARAT PERMANEN LARVA NYAMUK Culex sp. Onesiforus, Benaya Yamin; Setyoningrum, Fransisca Probo; San, Maria Pritinsia Cici
Jurnal Analis Laboratorium Medik Vol 10 No 1 (2025): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Proses pembuatan preparat larva nyamuk memiliki peran penting dalam dunia kesehatan untuk proses identifikasi. Salah satu tahap penitng dalam pembuatan preparat permanen larva nyamuk adalah tahap dehidrasi, yang dilakukan melalui perendaman larva kedalam alkohol. Banyaknya variasi pada prosedur perendaman etanol menjadikan belum adanya standar baku untuk proses dehidrasi dalam pembuatan preparat permanen larva nyamuk. Tujuan dari penelitian ini untuk mengetahui perbandingan 3 metode dehidrasi alkohol terhadap kualitas preparat permanen larva nyamuk. Penelitian ini merupakan penelitian eksperimental dengan menggunakan variasi dehidrasi etanol bertingkat. Jenis data yang diperoleh berupa data kategorik ordinal dan uji statistika yang digunakan dalam penelitian ini menggunakan uji Mann-Whitney U, pada kejernihan preparat didapatkan hasil, perlakuan alkohol 70% vs 30%,50%,96% nilai p = 0,609, alkohol 70% vs 60%,80%,90% nilai p=0,159, antar alkohol bertingkat = 30%,50%,96% alkohol bertingkat 60%,80%,90% didapatkan nilai p = 0,357. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan, tidak terdapat perbedaan yang signifikan antara metode perendaman alkohol terhadap kualitas preparat permanen lava nyamuk.
Co-Authors E. Elsa Herdiana Murhandarwati Lalangpuling, Indra E. San, Maria Pritinsia Cici Setyoningrum, Fransisca Probo Wijayanti, Mahardika A.