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All Journal Jurnal Biologi Tropis Berita Biologi
Ari Hepi Yanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Antibacterial Activity of Streptomyces sp. NrASA6 Culture Extract Isolated from Nypa Palm Worm Substrate against Escherichia coli, Staphylococcus aureus, and Aeromonas sp. NrBF9 Henno Wisnu Putra; Kurniatuhadi, Rikhsan; Tri Rima Setyawati; Ari Hepi Yanti
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6469

Abstract

Aquaculture is an effort to maintain aquatic commodities aimed at increasing cultivation. Probiotics are one of the components needed to serve as food additives that can prevent pathogenic bacterial infections in animals. One of the Actinomycetes species that has the potential to be used as a probiotic is Streptomyces sp. These species are known to be able to inhibit patogenic bacterial infections. The purpose of this research is to know about the antibacterial activity of the isolates Streptomyces sp. NrASA6 inhibiting the growth of Escherichia coli, Staphylococcus aureus, and Aeromonas sp. NrBF9, as well as the optimal for the test bacteria. The methods used in the antibacterial test are the agar diffusion method with pits and the swabbing method on the surface of medium MHA. The test treatment included incubation on time for Streptomyces sp. NrASA6, divided into 10, 15, 20, and 25 days. The tests were conducted at incubating temperatures of 30 °C and the diameter of the clear zone for 24 and 48 hours. The results of this research were that Streptomyces sp. NrASA6 made slowly for the patogenic bacterial Aeromonas sp. NrBF9 with a diameter of 16.97±1.15 mm for 24 hours, while an inhibition zone did not form on Escherichia coli and Staphylococcus aureus bacteri. The characteristic inhibiton of culture extract 15 days for Streptomyces sp. NrASA6 is bacteriostatic. The incubation period of Streptomyces sp. NrASA6 is influential in the stationary phase, namely 15th days after incubation.
KEPADATAN DAN POLA PERSEBARAN KELASI (Presbytis rubicunda) DI BUKIT BAKA TAMAN NASIONAL BUKIT BAKA BUKIT RAYA KALIMANTAN BARAT Pranadi, Resqi; Junardi; Ari Hepi Yanti; Marsaidi, Dodi
Berita Biologi Vol 24 No 2 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.4945

Abstract

Kelasi (Presbytis rubicunda) adalah salah satu spesies endemik pulau Borneo dan Karimata yang termasuk kedalam satwa liar dilindungi dan berstatus rentan (vulnerable). Berbagai ancaman terhadap kelasi dapat mempengaruhi kepadatan dan pola persebarannya. Tujuan dari penelitian ini untuk mengetahui kepadatan dan pola persebaran kelasi di jalur pendakian Bukit Baka kawasan Taman Nasional Bukit Baka Bukit Raya. Pengamatan kelasi menggunakan metode line transect distance sampling (LTDS) dengan variasi metode King. Analisis data untuk mendapatkan nilai kepadatan kelasi didapatkan menggunakan formula metode King sedangkan pola persebaran kelasi menggunakan formula indeks Morisita terstandarisasi. Pengambilan data dilakukan sepanjang 14,99 km dengan tiga kali ulangan yang mendapatkan 16 individu dengan jumlah individu paling banyak ditemukan pada pagi hari. Kepadatan individu kelasi didapatkan sebesar 6,7 individu/km2 dengan pola persebaran seragam.
GROWTH OF NYPA PALM WORMS (Namalycastis rhodochorde) JUVENILES FED ON NYPA PALM FRONDS FERMENTED BY CELLULOLYTIC BACTERIA NrLtC4 AND NrLtG2 Hosiana Patricia Sheren; Ari Hepi Yanti; Tri Rima Setyawati; Rikhsan Kurniatuhadi
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11352

Abstract

Nypa palm worms (Namalycastis rhodochorde: Polychaetes) play important ecological roles as detritivores of organic matter in mangrove ecosystems, bioindicators of water pollution, and sediment stabilizers in estuarine environments. N. rhodochorde also shows potential for use in fermented feed-based aquaculture due to its high nutritional value as a natural food source for aquatic organisms. Cellulolytic lactic acid bacteria isolated from the coelomic fluid and gastrointestinal tract of N. rhodochorde, identified as isolates NrLtC4 and NrLtG2, have potential as starter cultures for fermenting niypa palm fronds, which are used as feed in nypa palm worm cultivation. This study aimed to evaluate the growth of juvenile N. rhodochorde fed nypa palm fronds fermented by the cellulolytic bacteria NrLtC4 and NrLtG2. Juveniles were maintained under consistent stocking density, container size, and water volume, with three treatments arranged in a completely randomized design: unfermented nypa palm fronds (control), nypa palm fronds fermented with isolate NrLtC4, and nypa palm fronds fermented with isolate NrLtG2. The results demonstrated that fermented feed significantly influenced both the number of body segments [p = 0.00021 and 0.0028 (p < 0.05)] and body length [p = 0.0020 and 0.0035 (p < 0.05)] of the juveniles. Juveniles fed nypa palm fronds fermented with strain NrLtC4 showed increases of 29.6% in segment number and 58.5% in body length, while those fed fronds fermented with strain NrLtG2 exhibited increases of 36.6% and 63.9%, respectively. The highest juvenile survival rate was observed in the NrLtC4 fermented feed group at 72%, followed by 69.33% in the NrLtG2 group. These findings suggest that bacterial isolates NrLtC4 and NrLtG2 have promising potential as a consortium feed to enhance the growth of nypa palm worms in aquaculture.
Co-Authors Henno Wisnu Putra Hosiana Patricia Sheren Junardi Junardi Marsaidi, Dodi Pranadi, Resqi Rikhsan Kurniatuhadi, Rikhsan Tri Rima Setyawati