Agustono, Tridjoko
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Indol Acetic Acid Production of Indigenous Plant Growth Promotion Rhizobacteria from Paddy Soil Purwanto, Purwanto; Agustono, Tridjoko; Widjonarko, Bambang Rudianto; Widiatmoko, Teguh
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 7, No 1 (2019)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2019.087.1-7

Abstract

The aim of this research was to examine the diversity of indigenous plant growth promoting rhizobacteria from paddy soil and to obtain a superior isolate that can support the growth and vigor of rice plants. This research was conducted in the Laboratory of Agronomy and Horticulture, Faculty of Agriculture, Jenderal Soedirman University from July to September 2017. The bacteria were isolated from paddy soil rhizosphere originated from rice production centers of Banyumas Regency i.e. Kebasen, Rawalo, Patikraja, Jatilawang, and Karangwangkal. Results showed that indigenous PGPR from paddy soil were able to produce Indol Acetic Acid (IAA) in the range 0.05-5.40 ppm, but did not have the ability to solubilize phosphate. Plant Growth Promoting Rhizobacteria (PGPR) inoculation in rice seedlings was able to increase seed vigor, plant height, root length, and seed germination. Based on morphological and biochemical characters, PGPR isolates were identified as Bacillus sp., Pseudomonas sp., Streptococcus sp., and Staphylococcus sp.
PENGGUNAAN SITOKININ UNTUK MENGATASI CEKAMAN KEKERINGAN SELAMA FASE REPRODUKTIF TANAMAN KEDELAI Widiatmoko, Teguh; Agustono, Tridjoko; Faozi, Khavid
Agrin Vol 14, No 1 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.1.103

Abstract

Penelitian dilakukan untuk mengkaji efektifitas penggunaan sitokinin (kinetin) untuk menunda senesendaun tanaman kedelai yang mengalami kekeringan selama fase reproduktif dan pengaruhnya terhadap hasiltanaman. Percobaan dilakukan di rumah plastik Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokertomulai bulan Januari 2006 sampai April 2006. Rancangan perlakuan adalah faktorial (3x4) yang disusun dalamRancangan Acak Lengkap (RAL) dengan tiga ulangan. Faktor pertama berupa kadar air tanah yangmenggambarkan cekaman kekeringan selama fase reproduktif, yaitu kadar air tanah 100% kapasitas lapangan(KL-100%), kadar air tanah 75% kapasitas lapangan (KL-75%), dan kadar air tanah 50% kapasitas lapangan(KL-50%). Faktor kedua berupa tingkat konsentrasi zat pengatur tumbuh sitokinin (kinetin), yaitu konsentrasi0, 20, 40 dan 60 ppm. Pengamatan yang dilakukan meliputi kandungan air nisbi daun, kandungan klorofil daun,kehijauan daun, kandungan protein daun, laju transpirasi tanaman, jumlah polong, persentase polong isi, jumlahbiji, bobot biji per tanaman dan bobot 100 biji. Hasil penelitian menunjukkan cekaman kekeringan selama fasereproduktif tanaman kedelai secara umum menurunkan karakter fisiologi dan hasil tanaman kedelai. Kinetinefektif menunda senesen daun ditinjau dari kandungan klorofil dan protein daun. Penundaan senesen daun padatanaman kedelai yang mengalami kekeringan selama fase reproduktif ternyata justru berdampak negatif terhadappertumbuhan organ reproduktif.Kata kunci: kinetin, kekeringan, senesen, kedelai ABSTRACTThe research was designed to study cytokinin application to overcome the drought during reproductivestages of soybean. It was done in the plastic house Faculty of Agriculture, Jenderal Soedirman University,located in Purwokerto, Central Java from January 2006 up to April 2006. The experiment was a (3x4) factorialarranged in Completely Randomized Design (CRD) with three replications. The first factor was soil watercontent showing the level of drought, i.e. soil water content 100% field capacity, soil water content 75% fieldcapacity, and soil water content 50% field capacity. The second factor was the concentration of cytokinin(kinetin) i.e. 0, 20, 40 and 60 ppm. The observations were done on relative water content of the leaves,chlorophyll and protein content of the leaves, the level of leaves greenness, transpiration rate, number of podsand seeds, weight of seeds per plant and weight of 100 seeds. The result showed that the drought duringreproductive stages reduced on all physiological character and seed yield of soybean. Kinetin was effective todelay leaf senescence that observe on chlorophyll and protein content of leaves. Delaying leaf senescence duringthe drought at reproductive stages of soybean exactly influenced negatively on the growth of reproductiveorgans.Key words: kinetin, drought, senescence, soybean
PERTUMBUHAN DAN HASIL BEBERAPA GENOTIP KEDELAI BERBIJI BESAR PADA CEKAMAN KEKERINGAN DI BERBAGAI STADIA PERTUMBUHAN Widiatmoko, Teguh; Agustono, Tridjoko; Imania, Milla
Agrin Vol 16, No 1 (2012): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2012.16.1.129

Abstract

Budidaya kedelai kebanyakan dilakukan setelah musim tanam padi, sehingga tanaman kedelai seringkalimengalami cekaman kekeringan pada beberapa stadia kritis, seperti fase pembungaan dan pengisian polong.Penelitian ini dilaksanakan dengan tujuan untuk mengetahui potensi hasil dari galur harapan kedelai yangmemiliki karakter berbiji besar yang ditanam dengan cekaman kekeringan, mengetahui perbedaan responpertumbuhan dan hasil antar galur dan varietas tersebut terhadap cekaman kekeringan, dan saat pemberianperlakuan cekaman kekeringan yang masih dapat mendukung tanaman kedelai tumbuh dan berproduksi denganbaik.Penelitian ini telah dilakukan pada bulan Maret hingga Juni 2011 di rumah plastik Fakultas Pertanian,Universitas Jenderal Soedirman, untuk mengetahui pertumbuhan dan hasil genotip kedelai berbiji besar yaitugalur L/S:B6-G1, L/S:B6-G3, varietas Grobogan, Burangrang, dan varietas Agromulyo pada cekaman sebesar50% kapasitas lapang, yaitu: kontrol (100%), sejak 25 hari setelah tanam/sebelum berbunga/R1, dan sejak 50hari setelah tanam/sebelum pengisian polong/R5. Hasil penelitian menunjukkan bahwa Galur L/S:B6-G1merupakan genotip yang memiliki produksi paling baik dalam kondisi cekaman kekeringan 50% kapasitaslapang sejak 50 hst, yaitu 10,83 g biji per tanaman. Terdapat perbedaan respon antar genotip kedelai terhadapsaat terjadinya cekaman kekeringan, Galur L/S:B6-G1 dan Varietas Argomulyo merupakan genotip memilikiproduksi paling baik pada kadar air tanah 50% kapasitas lapang sejak 50 hst. Kedelai masih mampu berproduksibaik pada kadar air tanah 50% kapasitas lapang sejak 50 hst (fase pengisian polong/R5).Kata kunci: stadia pertumbuhan, kekeringan, kapasitas lapang, genotip, hasilABSTRACTMost of soybean cultivation was held after rice cultivating season, so that soybean crop often sufferingdrought stress on several critical stages of plant growth, such as at flowering and pod filling phase. Thisresearch aims to know the potential yield of big grains soybean line on drought stress, the growth and yield ofbig grains soybean line and varieties on drought stress, when the drought stress still able to support the soybeancrop to growth and produce well. Research was conducted in March until June 2011 at plastic house ofAgriculture Faculty, Jenderal Soedirman University to determine soybean genotypes growth and production i.e.L/S:B6-G1, L/S:B6-G3 line, Grobogan, Burangrang, and Agromulyo varieties under 50% of field capacity thatwere control (100%), since 25 days after planting/before flowering/R1, and 50 days after planting/before podfilling stage/R5. The results of the research showed that L/S:B6-G1 line has the best yield on 50% field capacitysince 50 days after planting, that was 10,83 g of seed per plant. There were different responses between soybeangenotypes and critical time of drought stress, L/S:B6-G1 line and Argomulyo were the best genotypes onproducing by 50% field capacity of soil moisture. Soybean crop was still able to produce well in soil watercontent that stressed 50% of field capacity since 50 days after planting (pod filling stage/R5).Key words: growth stages, drought, field capacity,genotype,yield