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All Journal Jurnal Kedokteran Brawijaya Majalah Kesehatan FKUB
Firdaningrum, Nimas Eka
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Medicine & Pharmacology Public Health

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HUBUNGAN ANTARA PENANDA sCD28 DAN sCTLA-4 TERHADAP PERSENTASE SEL LIMFOSIT T CD8+CD45RA+ PADA PENUAAN IMUN: - Handono, Kusworini; Susanti, Hani; Pratama, Mirza Zaka; Kalim, Handono; Wahono, Caesarius Singgih; Ria Famuji, Siti Roziah; Firdaningrum, Nimas Eka; Sumarta, Norma Hanifah; Rizky Fachry, Ade Wlidan
Majalah Kesehatan Vol. 10 No. 4 (2023): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/majalahkesehatan.2023.010.04.1

Abstract

Penuaan merupakan proses alami dan kompleks yang ditandai dengan penurunan fungsi fisiologis manusia sehingga berakibat pada perubahan sistem imun. Sampai saat ini, deteksi penuaan imun (immunosenescence) masih memiliki kendala yang berarti. Penanda satu-satunya yang diyakini sebagai prediktor penuaan imun adalah immune risk profile (IRP). Namun, IRP hanya dapat diamati dengan pemeriksaan flowsitometri yang membutuhkan alat canggih dan keterampilan khusus. Oleh karena itu, diperlukan penelitian yang menemukan suatu penanda deteksi penuaan imun melalui metode yang lebih mudah. Beberapa marker kostimulator memiliki bentuk terlarut di dalam serum. Penanda CD28 dan CTLA-4 pada membran memainkan peran utama pada regulasi aktivasi limfosit T dan marker diagnostik pada autoimun ataupun penuaan imun. Meskipun demikian, bentuk terlarut dari penanda tersebut masih belum banyak diteliti.  Tujuan dari penelitian ini adalah meneliti keterkaitan antara penanda sCD28 dan sCTLA-4 terhadap persentase IRP sel limfosit T CD8+CD45RA+ pada penuaan imun. Molekul kostimulator sCD28 dan sCTLA4 diukur menggunakan ELISA, sedangkan persentase sel limfosit T CD8+CD45RA+ menggunakan flowsitometri. Pada penelitian ini didapatkan 87 responden terdiri dari 23,0% responden kelompok individu muda, 40,2% kelompok pasien Lupus Eritematosus Sistemik (LES), dan 36,8% responden kelompok lansia. Kadar sCD28 dan sCTLA-4 terhadap persentase sel limfosit T CD8+CD45RA+ memiliki hubungan yang tidak signifikan pada penuaan imun. Pada penuaan imun, fungsi sel limfosit T yang mengekspresikan CD45RA menampilkan cacat proliferatif dan pensinyalan, tetapi tetap polifungsional.
The Role of Soluble Costimulatory Molecules as the Biomarkers for Aging Predictors Handono, Kusworini; Pratama, Mirza Zaka; Susianti, Hani; Wahono, Cesarius Singgih; Firdaningrum, Nimas Eka; Famuji, Siti Roziah Ria; Sumarta, Norma Hanifah; Rizky Fachry, Ade Wildan; Poetri, Levrita Nindya; Kalim, Handono
Jurnal Kedokteran Brawijaya Vol. 32 No. 3 (2023)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2022.032.03.4

Abstract

This study aimed to determine the role of the soluble costimulatory molecules in aging and the association with the presence of comorbid in aged individuals. Thirty-two elderly and twenty healthy subjects were included in this study. The soluble costimulatory molecules sCD28, sCD80, sCD86, sCD163, and sCTLA4 were measured using ELISA. The presence of comorbid was documented from medical records. Charlson Comorbidity Index (CCI) was measured to evaluate the survival/mortality risk for the subjects. The levels of the majority of soluble costimulatory molecules significantly increased in the elderly participants, while the level of sCD86 was comparable. There were weak positive correlations between the subject's age and levels of sCD28 (R=0.214, p=0.048), sCTLA4 (R=0.238, p=0.041), and sCD80 (R=0.317, p=0.012). sCD80 were discovered to be the best to predict immune aging in the elderly with AUC 0.71 [0.57-0.86], sensitivity 53,1%, specificity 80.0%, and cut off 129ng/ml. Most of the elderly participants had at least one comorbid, in which approximately 25.0% and 3.1% of the subjects were classified as mild and moderate CCI. Multivariate analysis showed that comorbidities in elderly individuals have been associated with levels of sCTLA4 ≥26.5ng/ml and sCD80 ≥129.0ng/ml. Furthermore, subjects with comorbid (CCI ≥1) were associated with sCD80 ≥129.0ng/ml (OR 12.44 [95% CI 1.32–117.03], p=0.027). Considering these results, sCD28, sCTLA4, and sCD80 can be developed as biomarkers for predicting immune aging and elderly comorbidities, respectively.
Co-Authors Famuji, Siti Roziah Ria Handono Kalim Hani Susanti, Hani Hani Susianti Kusworini Handono Poetri, Levrita Nindya Pratama, Mirza Zaka Ria Famuji, Siti Roziah Rizky Fachry, Ade Wildan Rizky Fachry, Ade Wlidan Sumarta, Norma Hanifah Wahono, Caesarius Singgih Wahono, Cesarius Singgih