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Effect of Different Concentrations and Combinations of Benzyl Aminopurine and Indole-3-Butyric Acid on Micropropagation of Vanilla Planifolia Khun, Sokhai; Heng, Chenda; Rien, Sothea; Rien, Sinet; Srean, Pao
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.90734

Micropropagation of explants in vitro has potency to address propagule demands for promoting large-scale vanilla production. Plant growth regulators (i.e., cytokinin, auxin) are necessary for the plant micropropagation success. Objective of this study is to determine the shoot multiplication and root development of Vanilla planifolia under the influence of different concentrations and combinations of BA and IBA for micropropagation. Sixty stem nodal segments of Vanilla planifolia were cultured on MS medium supplemented with IBA (0, 0.5, or 2.0 mg/L) and combined with BA (0, 0.5, 1.0, or 2.0 mg/L). Shoot multiplication and root induction were measured after 60 days of culture. The results show that the MS medium with 1 mg/L IBA hindered shoot growth, while the medium containing 1 mg/L BA yielded the highest number or weight of shoots per explant. For the root development, supplementing the medium with 0.5 mg/L or 1 mg/L IBA improved root length or number of roots per explant, respectively. This research establishes a valuable approach for vanilla micropropagation by utilising low concentrations of plant growth regulators and a rapid protocol. This paves the way for significant advancements in large-scale commercial production.

Investigating the Sri Lankan Cassava Mosaic Disease-related Characteristics of Cassava (Manihot esculenta Crantz) Nomura, Hisako; Baba, Tamon; Khin, Sophary; Pok, Panha; Than, Tha; Kim, Ok-Kyung; Natsuaki, Keiko; Srean, Pao; Ito, Kasumi
AGRITROPICA : Journal of Agricultural Sciences Vol. 7 No. 1 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.7.1.10-16

In this study, we aimed to investigate which components of cassava (Manihot esculenta Crantz) would be associated with the cassava tuber weight when infected with the Sri Lankan Cassava Mosaic Virus (SLCMV). We conducted multiple regressions to find associations between other cassava elements and tuber weights. This study was carried out in the Rukhak Kiri district in Battambang Province, located in northwestern Cambodia, in March 2019. We assessed two major varieties used in the region, namely, KU 50 and Rayong 7, using 90 samples—60 samples of KU 50 and 30 samples of Rayong 7. The results showed that the tuber weight of KU 50 was heavier than that of Rayong 7. In addition, the biomass of the stem, that is, the weight of all branches and leaves, were the sole strong factors associated with tuber weight for both KU 50 and Rayong 7. Moreover, as the tuber number declined, the tuber weight declined rapidly for Rayong 7. Overall, we concluded that SLCMV infection affects the biomass weight of cassava.

Agricultural Input Uses and Rice Productivity Assessment: A Case Study in Battambang and Svay Rieng Provinces, Cambodia Keo, Hak; Serey, Mardy; Yun, Sopheap; Srean, Pao
Journal of Social Knowledge Education (JSKE) Vol. 6 No. 3 (2025): August
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/jske.v6i3.1453

Purpose of the Study: The study aims to 1) assess the use of farming inputs and rice productivity in Battambang and Svay Rieng provinces, Cambodia; 2) identify the factors influencing rice productivity; and 3) predict rice productivity based on farming inputs. It seeks to provide insights into optimizing rice production and improving farmers' livelihoods. Methodology: A survey was conducted across 7 villages, 2 communes, and 2 districts in Battambang and Svay Rieng provinces, with 148 samples collected. Data were analyzed using quantitative and qualitative methods, including frequency analysis, descriptive statistics, cross-tabulation, chi-square tests, one-sample t-tests, and p-value probability. Main Findings: The study revealed that the average number of family members involved in farming was 2 (70%), and land sizes ranged from 2-4 hectares (40%). DAP was the most commonly used chemical fertilizer (66%). Land size significantly affected rice productivity (93%), followed by seed quality (86% yield increase) and pesticide use (91% yield increase). Chemical fertilizers had a near-total impact on productivity. The study recommends reducing chemical fertilizers in favor of organic alternatives for safer and higher-quality rice production. Novelty/Originality of This Study: This study provides a detailed analysis of farming inputs and their impact on rice productivity in Cambodia, emphasizing the role of land size, seed quality, and chemical inputs. It highlights the need for sustainable practices, such as organic fertilizers, and calls for stronger government and NGO support in agricultural policy and technical assistance. Future research could explore the impact of Good Agricultural Practices (GAP) on rice yield and quality.

Agricultural Input Uses and Rice Productivity Assessment: A Case Study in Battambang and Svay Rieng Provinces, Cambodia Keo, Hak; Serey, Mardy; Yun, Sopheap; Srean, Pao
Journal of Social Knowledge Education (JSKE) Vol. 6 No. 3 (2025): August
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/jske.v6i3.1453

Purpose of the Study: The study aims to 1) assess the use of farming inputs and rice productivity in Battambang and Svay Rieng provinces, Cambodia; 2) identify the factors influencing rice productivity; and 3) predict rice productivity based on farming inputs. It seeks to provide insights into optimizing rice production and improving farmers' livelihoods. Methodology: A survey was conducted across 7 villages, 2 communes, and 2 districts in Battambang and Svay Rieng provinces, with 148 samples collected. Data were analyzed using quantitative and qualitative methods, including frequency analysis, descriptive statistics, cross-tabulation, chi-square tests, one-sample t-tests, and p-value probability. Main Findings: The study revealed that the average number of family members involved in farming was 2 (70%), and land sizes ranged from 2-4 hectares (40%). DAP was the most commonly used chemical fertilizer (66%). Land size significantly affected rice productivity (93%), followed by seed quality (86% yield increase) and pesticide use (91% yield increase). Chemical fertilizers had a near-total impact on productivity. The study recommends reducing chemical fertilizers in favor of organic alternatives for safer and higher-quality rice production. Novelty/Originality of This Study: This study provides a detailed analysis of farming inputs and their impact on rice productivity in Cambodia, emphasizing the role of land size, seed quality, and chemical inputs. It highlights the need for sustainable practices, such as organic fertilizers, and calls for stronger government and NGO support in agricultural policy and technical assistance. Future research could explore the impact of Good Agricultural Practices (GAP) on rice yield and quality.

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