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All Journal Jurnal Biologi Tropis
Hayati, Rifda
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Study of The Distribution of Amorphophallus beccarii Engl in The Hiking Trail of Mount Singgalang Nature Park Hayati, Rifda; Vauzia, Vauzia
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6816

Abstract

Amorphophallus beccarii is one of the endemic plant of Sumatra, which has the characteristic of compound flowers, and emits a pungent odour that can attract insects such as flies and beetles to pollinate. Amorphophallu beccarii is found growing around the hiking trail of Mount Singgalang Nature Tourism Park, in connection with Mount Singgalang has been used as a Nature Tourism Park, it is feared that it will cause disruption of Amorphophallus beccarii habitat, in addition to the many human activities that carry out climbing activities, its interesting morphological shape is also the reason for the reduced population of Amorphophallus beccarii because it is collected as an ornamental plant. In conservation efforts, research has been carried out on Amorphophallus beccarii Engl Distribution Study in the Mount Singgalang Nature Tourism Park Hiking Trail. This research was conducted in February-March 2024. The research method used was stratified random sampling where sampling was carried out at an altitude of 1000-2000 mdpl along the hiking trail. Data were processed using the morisita index. The results showed that the distribution pattern of Amorphophallus beccarii on the Mount Singgalang hiking trail was clustered.
NGS-Based Metagenomic Approaches for Bacterial Community Analysis: A Review Hayati, Rifda; Marlina, Marlina; Agustien, Anthoni
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12426

Abstract

Microorganisms play a crucial role in biological, ecological, and biogeochemical processes; however, most environmental microorganisms cannot be readily grown under standard laboratory conditions, driving the development of Next-Generation Sequencing (NGS)-based metagenomic approaches that enable culture-independent characterization of microbial communities directly from environmental DNA. This review synthesizes the principles of NGS-based metagenomics, contrasts amplicon and shotgun approaches, compares major sequencing platforms and platform-selection criteria, and evaluates the applications and limitations of bacterial community analysis across the health, agricultural, and food sectors—an integration not commonly addressed together in previous reviews. Based on literature from 2018–2026 in PubMed/MEDLINE, Scopus, Web of Science, and Google Scholar, amplicon and shotgun metagenomics were found to offer complementary taxonomic and functional resolution, while platform choice depends on accuracy, read length, and budget. Across sectors, metagenomics supports pathogen surveillance and gut microbiome profiling in health, soil microbiome-based sustainability indicators in agriculture, and fermentation microorganism and antimicrobial resistance gene detection in food safety. Nevertheless, challenges remain regarding the accuracy of function prediction from amplicon-based data, bioinformatics pipeline standardization, and the validation of predicted microbial functions through complementary multi-omics approaches.
Co-Authors Anthoni Agustien Marlina Marlina Vauzia, Vauzia