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Effect of Extracellular Crude Protein (ECP) on expression IL-1, IL-6, IFN- in nile tilapia (Oreochromis niloticus) Herlina, Cucun; Fadjar, Mohammad; Maftuch, Maftuch; Yanuhar, Uun
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.43338

Abstract

Modern strategies incorporate preventive measures such as extracellular products, as soluble proteins with antigenic properties, as well as generating a broad immune response. The aim of research is to determine and analyze the effect of the extracellular product of E. tarda on enhancing the immunity of Nile tilapia (O. niloticus). The research design with treatment durations of d0, 3rd, 6th, 9th day, and control (A, B, C, D, K). ECP vaccination was injected intraperitoneally at a dose of 0.1 ml/fish. The parameters analyzed are the expression of IL-1, IL-6, and IFN-, phagocytic activity, leukocyte differential, total white blood cells, and relative percent survival. The results of phagocytosis activity were highest in treatment C at 84.18% 1.81. The highest leukocyte differential in treatment C was lymphocytes at 56% 2.00, monocytes at 28% 2.00, and 16% 0.00, with a total leukocyte count of 29 x 10^3 cells 2.64, and RPS values ranging from 83.33% to 100% over 9 days of post-vaccination maintenance. Then, post-vaccination, there was an increase in the expression levels of the cytokine genes IL-1 and IL-6, but there was no increase in the expression levels of the IFN- gene compared to the control in Nile tilapia. (O. niloticus).Keywords:ECPIL-1IL-6IFN-Immune Response
The Effect of Hermetia illucens Larvae on the Hematology of Tilapia (Oreochromis niloticus) Infected Edwardsiella tarda Tirani, Elisabeth; Maftuch, Maftuch; Fadjar, Mohammad; Awaluddin, Muhammad
Journal of Aquaculture and Fish Health Vol. 13 No. 1 (2024): JAFH Vol. 13 No. 1 February 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i1.36213

Abstract

Non-specific defense is the main defense in fish. One of the natural ingredients as a source immunostimulant isH. illucens larvae with a protein content of up to 30%. H. illucens larvae contains alkaloids, tannins, terpenoidsand saponins as immunostimulating agents. Immunostimulants are biological compounds that can boost theimmune system body. The purpose of this study is to find out the effect of H. illucens larvae in increasingimmunity non specific in tilapia (O. niloticus) which infected with E. tarda. In giving H. illucens larvae feed adlibitum with concentrations of A(30%), B(40%), C(50%), K(0%). In this research method, a completelyrandomized design (CRD) was used with 5 treatments and 3 replications in which each aquarium contained 10tilapia. Parameters tested are leukocytes, differential leukocytes, erythrocytes and hemoglobin. The resultsshowed that intake of 50% H. illucens larvae on tilapia could increase non-specific immunity, such as totalleukocytes, total erythrocytes, differential leukocytes, hemoglobin