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All Journal JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology) Jurnal Agripet
Purnomo, Nurul
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Agriculture, Biological Sciences & Forestry

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Study on The Production of Local Microorganisms (MOL) as a Starter for Cattle Feed Fermentation Aisyah, Nurul; Irwan, Muh; Purnomo, Nurul
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 11 No 1 (2025): Juni
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v11i1.224

Abstract

This study aims to determine the impact of various carbohydrate sources on the temperature, liquid volume, color, and pH of local microorganisms (MOL). The research employed a descriptive and quantitative approach, using a completely randomized design (CRD) with three treatments and six replicates. The treatments included potato (P1), stale rice (P2), and cassava (P3). Parameters measured were temperature, liquid volume, color, and pH. Results showed that the type of carbohydrate source significantly affected (P < 0.01) temperature, liquid volume, and pH. The highest temperature was observed in the P3 treatment, followed by P2 and P1. The greatest liquid volume occurred in P3, then P2 and P1. Treatment P1 had the highest pH level, followed by P2 and P3. The color of the fermented liquid remained similar to that of the raw materials. The findings indicated that the P3 treatment, involving cassava, produced optimal fermentation results, characterized by low pH levels, suitable temperatures, stable liquid volumes, and color closely resembling the original ingredients.
Polymorphisms of IGF-1 Gene in Indonesian Local Goat Reared Under Smallholder Farmers in Sulawesi Region Rahim, Lellah; Dagong, Muhammad Ihsan Andi; Bugiwati, Sri Rachma Aprilita; Purnomo, Nurul
Jurnal Agripet Vol 24, No 1 (2024): Volume 24, No. 1, April 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i1.28830

Abstract

ABSTRACT. The objective of this study was to determine the allele frequency of IGF-1 in Kacang and Peranakan Ettawa goats in Indonesia. The DNA samples were extracted from the blood of 105 heads of goats and collected from the South Sulawesi and West Sulawesi provinces. The IGF-1 target was amplified using the PCR-RFLP method. Two allele variants (A and B) and three genotypes of the IGF-1 gene (AA, AB, and BB) have been identified in the local goat population. Allele frequencies of IGF-1 were A (0.81) and B (0.19) in the total population of local goats. Allele A is the most common allele in both the Kacang and Peranakan Ettawa goat populations, with the highest frequency found in the Kacang population (0.87). The most common genotype is genotype AA, with the highest frequency in the Kacang population (0.75). The observed and expected heterozygosity were 0.276 and 0.303, respectively. The population of local goats in these regions was in Hardy-Weinberg equilibrium. The conclusion of this research is that the IGF-1 gene in local goats in the Sulawesi region is polymorphic and this diversity information can be used for association studies with growth traits, litter size, and twinning rate.(Polimorpisme gen IGF-1 pada populasi kambing lokal Indonesia yang dipelihara oleh peternak kecil di daerah Sulawesi)ABSTRAK. Penelitian ini bertujuan untuk mengetahui frekuensi alel IGF-1 pada kambing Kacang dan Peranakan Ettawa di Indonesia. Sampel DNA diambil dari darah 105 ekor kambing dan dikumpulkan dari Provinsi Sulawesi Selatan dan Sulawesi Barat. Target IGF-1 diamplifikasi menggunakan metode PCR-RFLP. Dua varian alel (A dan B) dan tiga genotipe gen IGF-1 (AA, AB, dan BB) telah diidentifikasi pada populasi kambing lokal. Frekuensi alel IGF-1 adalah A (0,81) dan B (0,19) pada total populasi kambing lokal. Alel A merupakan alel yang paling banyak ditemukan baik pada populasi kambing Kacang maupun kambing Peranakan Ettawa, dengan frekuensi tertinggi terdapat pada populasi Kacang (0,87). Genotipe yang paling umum adalah genotipe AA, dengan frekuensi tertinggi pada populasi Kacang (0,75). Heterozigositas yang diamati dan diharapkan adalah 0,276 dan 0,303, masing-masing. Populasi kambing lokal di wilayah ini berada dalam keseimbangan Hardy-Weinberg. Kesimpulan yang dihasilkan adalah bahwa gen IGF-1 pada kambing lokal yang dipelihara oleh peternak di wilayah Sulawesi bersifat polimorfik dan informasi keragaman ini dapat digunakan untuk studi asosiasi dengan sifat pertumbuhan, litter size dan kelahiran kembar.
Specific Species DNA Amplification as a Halal Authentication Method in Sausage Based on Genetic Markers Purnomo, Nurul; Mansur, Musdalifa; Nugraha, Angga; Dagong, Muh. Ihsan A.; Natsir, Asmuddin
Jurnal Agripet Vol 23, No 2 (2023): Volume 23, No. 2, Oktober 2023
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v23i2.26595

Abstract

ABSTRACT. Sausage is a processed meat food that is susceptible to counterfeiting by mixing non-halal meat, so a valid, fast and cheap halal authentication method is needed for sausages. This study aims to determine the specific species genes of Cytochrome-b in target DNA amplification as a halal authentication method in sausages. In this study, the samples used were beef sausage, chicken sausage, and pork sausage. The stages of this research consisted of DNA purification, measuring DNA quality, amplifying object DNA using Cytochrome-b, electrophoresis, and visualization of the gel documentation. The DNA purification results from beef sausage, chicken sausage, and pork sausage respectively obtained concentrations of 3 (ng/l), 2.6 (ng/l), and 2.8 (ng/l), and purity of 1.15, 0.76, and 0.88. Meanwhile, DNA amplification produced fragments with lengths for beef sausage, chicken sausage, and pork sausage, namely the Cytochrome-b gene 274 bp, 227 bp, and 398 bp. Based on this study, it's concluded that the Cytochrome-b genes could amplify target DNA from beef sausage, chicken sausage, and pork sausage so that they could be used as gene markers for authenticating halal sausages.(Amplifikasi DNA Spesifik Spesies sebagai metode autentikasi halal pada sosis berdasarkan penanda genetic)ABSTRAK. Sosis merupakan makanan olahan daging yang rentan terhadap pemalsuan dengan mencampurkan daging non halal, sehingga diperlukan metode autentikasi halal yang valid, cepat dan murah untuk sosis. Penelitian ini bertujuan untuk mengetahui gen spesies spesifik Cytochrome-b pada amplifikasi DNA target sebagai metode autentikasi halal pada sosis. Dalam penelitian ini, sampel yang digunakan adalah sosis sapi, sosis ayam, dan sosis babi. Tahapan penelitian ini terdiri dari pemurnian DNA menggunakan Quick-DNA Plus Kits, Zymo Research, pengukuran kualitas DNA, amplifikasi pada DNA target menggunakan gen Cytochrome-b, elektroforesis, dan visualisasi pada gel dokumentasi. Hasil pemurnian DNA sosis sapi, sosis ayam, dan sosis babi masing-masing diperoleh konsentrasi 3 (ng/l), 2,6 (ng/l), dan 2,8 (ng/l), dan kemurnian 1,15, 0,76, dan 0,88. Sedangkan amplifikasi DNA menghasilkan fragmen dengan panjang untuk sosis sapi, sosis ayam, dan sosis babi yaitu gen Cytochrome-b 274 bp, 227 bp, dan 398 bp. Berdasarkan penelitian ini, disimpulkan bahwa gen Cytochrome-b dapat mengamplifikasi DNA target dari sosis sapi, sosis ayam, dan sosis babi sehingga dapat digunakan sebagai penanda gen untuk autentikasi sosis halal.
Co-Authors Angga Nugraha Asmuddin Natsir Dagong, Muh. Ihsan A. Mansur, Musdalifa Muh Irwan, Muh Muhammad Ihsan Andi Dagong Nurul Aisyah Rahim, Lellah SRI RACHMA APRILITA BUGIWATI