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All Journal Jurnal Ilmu Pangan dan Hasil Pertanian Jurnal Leverage, Engagement, Empowerment of Community (LeECOM) Stannum : Jurnal Sains dan Terapan Kimia
Yoewono, Jessica Renata
Department Of Food Technology, Universitas Ciputra Surabaya, Region Citraland CBD Boulevard Made, Surabaya, Jawa Timur, 60219
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Public Health Social Sciences Other

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A review of the development of Polymerase Chain Reaction technique and its uses in Scientific field Lucyenne Angeline Giri Putra; Christianus Jodi Yonathan; Nabila Imania Niedhatrata; Mochammad Hilmy Rizka Firdaus; Jessica Renata Yoewono
Stannum : Jurnal Sains dan Terapan Kimia Vol 2 No 1 (2020): April
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v2i1.1619

Abstract

PCR (Polymerase Chain Reaction) is a scientific term and technique in molecular biology which able to generate copies of a specific DNA from two short oligodeoxynucleotide sequences (also called primers) by a polymerase-dependent repetitive thermal reaction. PCR technique brought a revolution in science especially in molecular biology since its first discovery back in 1984 as its simplicity and not a time-consuming trait. Over the past decades, PCR techniques have been modified to make it suitable for the application in each scientific field. PCR nowadays is involved in almost all studies that required DNA fragments manipulation including in food and medical analysis. By taking advantage of this revolutionary technique and if developed and used well, it would become very beneficial for humanity in many aspects
PENGEMBANGAN INOVASI KECAP AROMATIK BEBAS AFLATOKSIN DI DESA BALONG TUNJUNG, KECAMATAN BENJENG, KABUPATEN GRESIK Sulistyo, Joko; Dewi, Irra Chrisyanti; Yoewono, Jessica Renata
Jurnal Leverage, Engagement, Empowerment of Community (LeECOM) Vol. 4 No. 2 (2022): Jurnal Leverage, Engagement, Empowerment of Community (LeECOM)
Publisher : Universitas Ciputra Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/leecom.v4i2.3503

Abstract

Untuk mencegah kontaminasi aflatoksin dalam produksi kecap tradisional di Indonesia, ragi kecap dari biakan kapang mutan Aspergillus oryzae yang berspora putih, dipersiapkan dari biakan kapang induk A. oryzae yang berspora hijau, melalui teknik iradiasi dengan sinar UV pada intensitas tertentu, sehingga diperoleh ragi kecap mutan yang tidak memproduksi aflatoksin dan dapat digunakan sebagai ragi kecap dalam rangka pengembangan produk kecap aromatik bebas aflatoksin di Desa Balong Tunjung, Kecamatan Benjeng, Kabupaten Gresik. Proses pembuatan kecap aromatik bebas aflatoksin dilakukan melalui tiga tahapan utama, yaitu fermentasi ragi (koji) yang dipersiapkan melalui inokulasi ragi kecap mutan pada kacang kedelai yang telah direndam, dikuliti dan dimasak selama 3–5 hari, dilanjutkan dengan fermentasi garam (moromi) berupa perendaman koji pada kadar garam tinggi (18–22%) selama 1–6 bulan, dan selanjutnya dilakukan pengolahan moromi dengan penambahan rempah-rempah aromatik serta pengemasan produk kecap. Hasil fermentasi koji, menunjukkan pertumbuhan miselia kapang berspora putih secara merata pada permukaan kedelai, menunjukkan proses penguraian secara enzimatik protein secara optimal, sehingga mempercepat proses penguraian bahan-bahan organik yang terkandung dalam kedelai selama fermentasi moromi, sehingga meningkatkan kualitas nutrisi. Hasil tersebut menunjukkan bahwa penggunaan ragi kecap mutan berspora putih tidak hanya berkontribusi pada pencegahan kontaminasi aflatoksin tetapi juga meningkatkan kualitas nutrisi pada produk akhir kecap.
Acceptance Test of Jokowi's Herbal Beverage with Antioxidant Properties in Isotonic Beverage Formulation Yoewono, Jessica Renata; Krisbianto, Oki; Sugiharto, Yurika; Ridfan, Lorent Permata
Jurnal Ilmu Pangan dan Hasil Pertanian Vol 7, No 2 (2023)
Publisher : Program Studi Teknologi Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/jiphp.v7i2.17288

Abstract

A herbal beverage mixture based on extracts of turmeric (Curcuma longa), Javanese turmeric (Curcuma xanthorrhiza), and red ginger (Zingiber officinale) with a ratio of 8:6:3 was developed as an isotonic beverage after describing its sensory characteristics and antioxidant properties. Three different dilutions (1:10, 1:15, and 1:20) were used for sensory acceptance testing by consumers. Antioxidant activity tests using DPPH followed by HPLC were conducted on each extract as well as the most concentrated dilution and the best treatment. The sensory testing results indicated that the 1:20 dilution treatment was the most acceptable. The DPPH results showed that the 1:10 and 1:20 dilutions had inhibition activities of 52.34% and 37.94% respectively, which correlated with the quantification of curcumin using HPLC, namely 167.47 µg/g and 125.06 µg/g. The higher the curcumin content, the higher the DPPH inhibition activity. Atomic absorption spectroscopy (AAS) analysis revealed that the Na and K content in the 1:20 diluted herbal beverage were 11.66 mg/kg and 13.85 mg/kg respectively, which were still lower than the standard SNI-01-4452-1998. Therefore, the isotonic beverage was formulated by adding low-sodium salt and sodium citrate as sources of sodium, potassium, and citrate.
Co-Authors Christianus Jodi Yonathan Florence Pribadi Imanuel D. K. Aji Irra Chrisyanti Dewi Joko Sulistyo Lorent P. Ridfan Lucyenne Angeline Giri Putra Mitha A. P. Handojo Mochammad Hilmy Rizka Firdaus Muliasari Kartikawati Nabila Imania Niedhatrata Oki Krisbianto Ridfan, Lorent Permata Sugiharto, Yurika