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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Sumberdaya Hayati
Winnie, Ruth Martha
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Perilaku Defensif Lebah Apis cerana Terhadap Stimulus Isopentil Asetat (IPA): Honey bee Apis cerana Defensive Behavior based on Isopentyl Acetate (IPA) Stimulus Winnie, Ruth Martha; Atmowidi, Tri; Raffiudin, Rika
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.238-244

Abstract

Honey bee workers perform colony defense behavior to maintain colony integrity against predators. The aim of this research was to explore the response of A. cerana defensive behavior by using Isopentyl Acetate (IPA) as the stimulus which is the main volatile compound in the venom. Five concentrations of IPA volume i.e. 10, 20, 30, 40, and 50 µL IPA were used as the stimulus and each volume was pipetted to a cotton ball. The cotton ball was placed 10 cm in front of the hive entrance in oscillatory motion for five minutes. Natural defensive behavior of A. cerana (balling behavior), balling temperature and A. cerana predator were observed. Observation of defensive behavior was conducted by using colonies A. cerana obtained from Parung Panjang, Bogor and Nyalidung, Sukabumi; and using colonies A. cerana obtained from Sumedang and Banjarsari, Lebak. Based on the research, five A. cerana defensive behavior responses were observed, i.e.: guarding in front of bee hive entrance, recruitment at the hive entrance, fly toward the cotton ball, balling around the hive entrance, and balling on cotton ball. A. cerana showed defensive behavior due to IPA stimulus, however, there was no significant difference (P>0.05) with the variety IPA stimulus volume. We found the temperature of 44°C inside the natural balling of A. cerana defense behavior, as the colony defense mechanism in response to the natural predator Vespa sp. wasp.
INTRASPECIFIC VARIATIONS OF 16S MITOCHONDRIAL GENE SEQUENCES OF YELLOW RICE STEM BORER,scirpopbaga incertulas (LEPIDOPTERA: CRAMBIDAE) FROM WEST JAVA RAFFIUDIN, RIKA; WINNIE, RUTH MARTHA; SAMUDRA, I MADE
BIOTROPIA Vol. 18 No. 1 (2011): BIOTROPIA Vol. 18 No. 1 June 2011
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2011.18.1.135

Abstract

Yellow rice stem borer (Scirpophaga incertulas) is one of the most important rice pest insects in Asia, including Indonesia. However, there is a lack of genetic data for this important agricultural insect.  Therefore, this study was conducted to explore intraspecific differentiation of S. incertulas partial 16S mitochondrial gene from Bogor, Karawang, Indramayu and Cirebon (West Java, Indonesia). Here, we reported a total of 325 bp of 16S mitochondrial gene of S. incertulas from the obtained samples. Among all DNA sequences, three haplotypes of 16S mitochondrial gene were observed and submitted to GenBank under Accession Number of GU191881, GU191882, GU191883, respectively for haplotype 1, 2, and 3.  The haplotype 1 was found in all S. incertulas surveyed locations, except Bogor. The haplotype 2  and 3 were found only in from Cirebon and Bogor samples. These haplotype variations can be applied as DNA markers for S. incertulas early larva detection method among other rice stem borers. Hence, further explorations of the mitochondrial variations of S. incertulas in Java and other parts of Indonesia are needed.   Keywords: moth, haplotypes, genetic differentiations,  molecular identification
Co-Authors I Made Samudra RIKA RAFFIUDIN Tri Atmowidi