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All Journal Jurnal Ilmu Pertanian Indonesia AGROLOGIA : Jurnal Ilmu Budidaya Tanaman
J. K. J. Laisina
Jurusan Budidaya Pertanian, Fakultas Pertanian, Universitas Pattimura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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The In Vitro Screening of Drought Tolerant Potatoes (Solanum tuberosum L) of the Center for Tropical Horticulture Studies - IPB collections Jane Kathryne Jolanda Laisina; Awang Maharijaya; Sobir Sobir; Agus Purwito
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 2 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.2.235

Abstract

In supporting the replacement of potato varieties with the varieties that are adaptive to drought, effective and fast methods are needed. One of which is through in vitro screening of drought-tolerant genotypes. The aims of this research were to determine the appropriate concentration of sorbitol as a critical limit in the in vitro screening process of drought-tolerant genotypes, as well as to determine vegetative characteristics that could be used as indicators of in vitro selection and also to obtain in vitro drought-tolerant genotypes. To achieve these objectives, we used single-nodal explants of seven genotypes cultured for six weeks on MS media added with four levels of sorbitol concentration. Of the four treatments, sorbitol level that could distinguish susceptible and tolerant plants was 0.2 M sorbitol and at this concentration, the characteristics that showed significant interactions were plant height and root length, so that both characteristics could be used as selection indicators for the analysis of tolerance tests for the seven genotypes. The results of tolerance test using the analysis of relative decline and stress-susceptibility index (SSI) showed that PKHT4 and PKHT6 were the genotypes that were tolerant in vitro. Keywords: drought tolerant, in vitro, Solanum tuberosum, sorbitol
Pelestarian Secara In Vitro Melalui Metode Pertumbuhan Lambat Pada Beberapa Genotipe Ubi Jalar (Ipomea Batatas (L) Lam) Jean K J Laisina
Agrologia Vol 2, No 2 (2013)
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v2i2.267

Abstract

The objectives of this research were to estimate responses of several sweet potato genotypes in preservation media through in vitro slow growth preservation, and to achieve cheap and accessible media. Experiment was arranged in a completely randomized design with a single factor. The experimental factor was sweet potato genotype (Sukuh, 421.34,343.15 and 2040.8). These genotype were tested in a preservation media which consisted of 15% coconut water +  30 mg/l aspirin + 50 g/l sucrose + 7 g/l agar. Experiment was replicated four times. Data was analyzed parametrically and non-parametrically. The result showed that genotype gave high responses to preservation media. Genotype Sukuh produced highest leaves while genotype 421.34 yielded highest numbers of root and internode. In the preservation medium of Genotype Sukuh through in vitro slow growth preservation, aspirin could be added to inhibit growth by increased leaf senesence. This experiment also showed that Hiponex (20:20:20) could be used as the basic media for in vitro preservation of sweet potato
Konsentrasi Sukrosa Dan Agar Di Dalam Media Pelestarian In-Vitro Ubi Jalar Var. Sukuh Jane K. J Laisina
Agrologia Vol 2, No 1 (2013)
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v2i1.279

Abstract

The objective of this research was  to obtain suitable concentration of sucrose and agar in the sweet potato (Ipomea batatas (L) Lam) in vitro preservation medium, in order to make plant grow slowly and healthy. Experiment was done in Molecular Biologi Laboratory of PAU IPB. The experiment was arranged in factorial complete random design, using sweet potato var. Sukuh in order to know the effect of sucrose (40,50,60,70 and 80 g l-1) and agar (7 and 8 g l-1) which were added 1 g l-1 hyponex fertilizer 20-20-20. The experiment was replicated four times. Data were analyzed parametrically and non-parametrically. The result of these experiments showed the suitable conservation media was 1 g l-1 hyponex 20-20-20 + 60 g l-1sucrose + 7 g l-1 agar. Threfore, the media composition MS could produce high green inter nodes number, high root number and more than two green leaf number. This experiment also showed that MS media could be replaced by a cheaper and easily found conservation medium.
Co-Authors Agus Purwito Awang Maharijaya Sobir Sobir