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All Journal Jaringan Laboratorium Medis International Journal of Community Service
Rachmad Bayu Kuncara
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Religion Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Public Health Social Sciences Other

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Desain Primer untuk Deteksi Gen Diphtheria Toxin Repressor (dtxR) sebagai Biomarker Bakteri Corynebacterium diphtheriae Menggunakan In Silico PCR Hilari Rio Rosa Nastiti; Rachmad Bayu Kuncara
Jaringan Laboratorium Medis Vol 5, No 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.10588

Abstract

Corynebacterium diphtheriae is the bacteria that causes diphtheria. The virulence factor of C. diphtheriae comes from the bacteria's ability to produce bacterial toxins. Toxin production is regulated by a set of genes called tox/dtx genes and is regulated by the dtxR gene. The aim of this study was to design primers used to evaluate the dtxR gene using bacterial DNA sequences. This research is experimental research with a literature study approach using the In silico Polymerase Chain Reaction (PCR), NCBI (National Center for Biotechnology Information), Primer3Plus, and Oligo Calculator applications. The sample obtained from genbank NCBI was C. diphtheriae dtxR gene M80337.1. In silico PCR examination was carried out using newly designed primers from Primer3Plus with 50 genomic DNA of Corynebacterium spp. taken from the In silico PCR database. The dtxR primer pair: '5-ACAGTTAGCCAAACCGTTGC-3' and 5'-TGCGTTCAACTTCGTCACTC-3' can produce a single DNA amplicon measuring 226 bp specifically for C. diphtheria types and no amplicon bands were generated from other Corynebacterium genomes. Based on the study results, this pair of specific primers can be used for in vitro PCR testing and can be used to develop rapid detection of diphtheria.
Socialization Of Clean And Healthy Living Behaviors And Screening Of Non-Communicable Diseases In Efforts To Prevent The Pandemic Covid-19 Kahar, Fitriani; Devi Etivia Purlinda; Djoko Priyatno; Ichsan Hadipranoto; Rachmad Bayu Kuncara
International Journal Of Community Service Vol. 1 No. 1 (2021): May 2021 (Indonesia - Malaysia )
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijcs.v1i1.6

Abstract

Coronavirus (COV) is a large family of viruses that cause a slaughter starting from mild to severe symptoms. The disease caused by this virus is called Covid-19. One effort in the prevention of Covid-19's penis is to hold a health protocol and get clean life behavior and sehāt in everyday life. Market Ganesha is one of the traditional markets that functions for buying and selling facilities where many people gather so that it becomes a means of repentance of Covid-19 if the community is a PHB pedagang and not applied health protocols. From the results of the activity it was seen that the community was enthusiastic in participating in the socialization of PHBS and alternately to carry out glucose blood pressure. This socialization activity is not only explained but but also socialization of Gerdik and behavior and obedient to the efforts of nitrance. This activity also completes one of the destinations, namely members of the health examination of residents in the form of blood pressure checks and laboratories (blood glucose examination). New) With the majority of respondents are in the category of 41-50 years and the majority of respondents with female sex. The conclusion is that the trader's society has a risk factor for suffering from the disease not menulus (PTM) especially and diabetes mellitus.
Co-Authors Devi Etivia Purlinda Djoko Priyatno Hadipranoto, Ichsan Hilari Rio Rosa Nastiti Kahar, Fitriani